Asian Journal of Bio Science

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Purification and Characterization of Riboflavin Carrier Protein from Hen Egg-Yolk and Pigeon Egg-Yolk


Affiliations
1 Department of Biochemistry, N. R. I. Medical College, CHINA KAKANI, (A.P.), India
2 Department of Chemistry, Univ. Arts & Science College, Kakatiya University, Warangal, (A.P.), India
3 Department of Chemistry, Kakatiya University, Warangal, (A.P.), India
     

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Riboflavin carrier protein (RCP) has been purified from both pigeon and hen egg- yolk by ion exchange chromatography and gel filtration techniques. Purity of pigeon egg- yolk and hen egg-yolk RCPs are determined by SDS-PAGE. Their structures are compared using Circular Dichroism (cd) Spectra and Fluorescence Spectra. For Pigeon egg-yolk RCP amino acid composition was analyzed. In the present study it is observed that the two proteins have similar secondary structure but they differ in their tertiary structures. The amino acid composition of pigeon egg-yolk RCP has close similarity with hen egg-yolk RCP. This study suggests the existence of some significant structural difference between RCPs from pigeon and hen.

Keywords

Riboflavin Carrier Protein, Protein Purification and Characterization.
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  • Purification and Characterization of Riboflavin Carrier Protein from Hen Egg-Yolk and Pigeon Egg-Yolk

Abstract Views: 257  |  PDF Views: 0

Authors

R. Vijaya Lakshmi
Department of Biochemistry, N. R. I. Medical College, CHINA KAKANI, (A.P.), India
J. Madhukar
Department of Chemistry, Univ. Arts & Science College, Kakatiya University, Warangal, (A.P.), India
M. S. K. Prasad
Department of Chemistry, Kakatiya University, Warangal, (A.P.), India

Abstract


Riboflavin carrier protein (RCP) has been purified from both pigeon and hen egg- yolk by ion exchange chromatography and gel filtration techniques. Purity of pigeon egg- yolk and hen egg-yolk RCPs are determined by SDS-PAGE. Their structures are compared using Circular Dichroism (cd) Spectra and Fluorescence Spectra. For Pigeon egg-yolk RCP amino acid composition was analyzed. In the present study it is observed that the two proteins have similar secondary structure but they differ in their tertiary structures. The amino acid composition of pigeon egg-yolk RCP has close similarity with hen egg-yolk RCP. This study suggests the existence of some significant structural difference between RCPs from pigeon and hen.

Keywords


Riboflavin Carrier Protein, Protein Purification and Characterization.