Asian Journal of Bio Science

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Validation of Bt-gene and Study of Packaging Materials on Seed Longevity in Bt-cotton Hybrids


Affiliations
1 Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
     

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A laboratory experiment was carried out to validate Bt gene and to study the storage potential of Bt-cotton hybrids. Results revealed that, all the six Bt-cotton hybrid seed lots selected for the study were positive for Bt-gene (Cry 1 Ac). In the storage study, the seed lot L1 recorded highest germination (71.55%) and lowest in L3 (68.11%) at the end of ten months of storage. Among the packaging materials, seeds stored in P1 and P2 recorded highest germination (71.50 and 71.50%, respectively) compared to P3 (68.11 %). Seedling vigour index was highest (2007) in L1 and lowest (1792) in L6. Irrespective of seed lots, P1 recorded highest vigour (1981) followed by P2 (1963) and lowest in P3 (1759) at the end of ten months of storage. Germination per cent in polythene bag and polylined cloth was above the minimum seed certification standards (70.00%) upto ten months of storage. However, genotypic differences were observed with respect to seed quality.

Keywords

Cottton, Validation, Bt Gene, Storability, Seed Quality
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  • Validation of Bt-gene and Study of Packaging Materials on Seed Longevity in Bt-cotton Hybrids

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Authors

G. K. Ranganath
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
P. Balakrishna
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
C. K. Pramila
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
B. C. Chennakeshava
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
R. Paramesh
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India

Abstract


A laboratory experiment was carried out to validate Bt gene and to study the storage potential of Bt-cotton hybrids. Results revealed that, all the six Bt-cotton hybrid seed lots selected for the study were positive for Bt-gene (Cry 1 Ac). In the storage study, the seed lot L1 recorded highest germination (71.55%) and lowest in L3 (68.11%) at the end of ten months of storage. Among the packaging materials, seeds stored in P1 and P2 recorded highest germination (71.50 and 71.50%, respectively) compared to P3 (68.11 %). Seedling vigour index was highest (2007) in L1 and lowest (1792) in L6. Irrespective of seed lots, P1 recorded highest vigour (1981) followed by P2 (1963) and lowest in P3 (1759) at the end of ten months of storage. Germination per cent in polythene bag and polylined cloth was above the minimum seed certification standards (70.00%) upto ten months of storage. However, genotypic differences were observed with respect to seed quality.

Keywords


Cottton, Validation, Bt Gene, Storability, Seed Quality

References