Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Validation of Bt-gene and Study of Packaging Materials on Seed Longevity in Bt-cotton Hybrids


Affiliations
1 Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
     

   Subscribe/Renew Journal


A laboratory experiment was carried out to validate Bt gene and to study the storage potential of Bt-cotton hybrids. Results revealed that, all the six Bt-cotton hybrid seed lots selected for the study were positive for Bt-gene (Cry 1 Ac). In the storage study, the seed lot L1 recorded highest germination (71.55%) and lowest in L3 (68.11%) at the end of ten months of storage. Among the packaging materials, seeds stored in P1 and P2 recorded highest germination (71.50 and 71.50%, respectively) compared to P3 (68.11 %). Seedling vigour index was highest (2007) in L1 and lowest (1792) in L6. Irrespective of seed lots, P1 recorded highest vigour (1981) followed by P2 (1963) and lowest in P3 (1759) at the end of ten months of storage. Germination per cent in polythene bag and polylined cloth was above the minimum seed certification standards (70.00%) upto ten months of storage. However, genotypic differences were observed with respect to seed quality.

Keywords

Cottton, Validation, Bt Gene, Storability, Seed Quality
Subscription Login to verify subscription
User
Notifications
Font Size


  • Abdelmagid, A.S. and Osman, A.M. (1973). Influence of storage period and temperature on viability and chemical composition of cotton seeds. Ann. Bot., 39: 237-248.

  • Abdul-Baki, A.A. and Anderson, J.D. (1973). Vigour determination in soybean seeds by multiple criteria. Crop Sci., 13: 639-633.

  • Bishnoi, U.R. and Delouche, J.C. (1980). Relationship of vigour tests and seed lots to cotton seedling establishment. Seed Sci. & Technol., 8: 341-346.

  • Delouche, J.C. (1973). Seed maturation. In: Seed production manual. NSC and Rockfeller Foundation. pp. 162-165.

  • Doijode, S.D. (2002). Seed storage of horticultural crops. CBS publishers and distributors, pp. 269-273.

  • Freitas, R.A., Dias D.C.F.S., Cecon, P.R., Reis, M.S. and Lias, Dias (2002). Storability of cotton seeds predicted by vigour tests, Seed Sci. & Technol., 30: 403-410.

  • Goel, Anuradha, Goel, Ajay Kumar and Sheoran, Inder Singh (2003). Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J. Pl. Physiol., 160: 1093-1100.

  • ISTA (2007). International rules for seed testing, Ed. 2007, ZURICH (SWITZERLAND).

  • Meena, R.A., Rathinavel, R.K., Deshmukh, R.K. and Tuteja, O.P. (1998). Storage potential of tetraploid and diploid cottons under ambient conditions, Seed Res., 27(1): 125-127.

  • Randhawa, G.J. (2005). Detection methods for GMOs/LMOs, NRC on DNA fingerprinting, NBPGR, NEW DELHI (INDIA).

  • Selvaraj, J.A. and Ramaswamy, K.R. (1977). Studies on seed quality as influenced by picking in cotton cv.MCU, Madras Agric. J., 65: 658-663.

  • Shivayogi Ryavalad, Biradar Patil, N.K., Girradi, R.S. and Katageri, I.S. (2009). Effect of acid delinting seed treatment and containers on storability of cotton hybrid. Karnataka J. agric. Sci., 22(1): 56-60.

  • Snedecor, G.W. and Cochran, W.G. (1967). Statistical methods, The IOWA State University Press, USA, pp.593.

  • Wood, D.W., Longeden, P.C. and Scoot, R.K. (1977). Seed size variation, its extent, source and significance in field crops. Seed Sci. & Technol., 5: 337-352.


Abstract Views: 387

PDF Views: 0




  • Validation of Bt-gene and Study of Packaging Materials on Seed Longevity in Bt-cotton Hybrids

Abstract Views: 387  |  PDF Views: 0

Authors

G. K. Ranganath
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
P. Balakrishna
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
C. K. Pramila
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
B. C. Chennakeshava
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
R. Paramesh
Department of Seed Science and Technology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India

Abstract


A laboratory experiment was carried out to validate Bt gene and to study the storage potential of Bt-cotton hybrids. Results revealed that, all the six Bt-cotton hybrid seed lots selected for the study were positive for Bt-gene (Cry 1 Ac). In the storage study, the seed lot L1 recorded highest germination (71.55%) and lowest in L3 (68.11%) at the end of ten months of storage. Among the packaging materials, seeds stored in P1 and P2 recorded highest germination (71.50 and 71.50%, respectively) compared to P3 (68.11 %). Seedling vigour index was highest (2007) in L1 and lowest (1792) in L6. Irrespective of seed lots, P1 recorded highest vigour (1981) followed by P2 (1963) and lowest in P3 (1759) at the end of ten months of storage. Germination per cent in polythene bag and polylined cloth was above the minimum seed certification standards (70.00%) upto ten months of storage. However, genotypic differences were observed with respect to seed quality.

Keywords


Cottton, Validation, Bt Gene, Storability, Seed Quality

References