![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_open_medium.gif)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_restricted_medium.gif)
Screening of Diazotrophic Bacterial Communities from Wild Rice (Oryza indica) and Cultivated Rice (Oryza sativa) and their Plant Growth Promoting Activities
Subscribe/Renew Journal
A study was undertaken to screen the free living, culturable diazotrophic bacteria from wild rice (Oryza indica) and cultivated rice (Oryza sativa) and their plant growth promoting activities. Out of forty eight diazotrophic isolates, thirty eight isolates recorded positive growth in Nfree medium which were further analyzed for total nitrogen and ammonia. Based on total nitrogen and ammonia production, twenty eight diazotrophic isolates were selected for nitrogenase activity. The highest nitrogenase activity was exhibited by isolate GDR16 (413456.6 nm of ethylene mg-1 protein-1 hr). For PGPR activity 11 isolates from O.indica and 9 isolates from O.sativa with elite nitrogenase activity were selected for PGPR as well as mineral solubilization studies. Out of these 20 diazotrophic isolates, 11 isolates showed IAA production. The maximum amount of IAA was produced by CBE1 (35.5 1.14g ml-1). The highest amount of GA was produced by GDR13 (21.70.19 g ml-1), followed by GDR 7 which produced 18.40.23 g ml-1. The maximum siderophore production was recorded with CBE1 (43.94 0.64g mg-1 dry weight of cell of catechol type). With respect to mineral solubilization, 17 were able to solubilze the insoluble phosphorus and 7 were able to solubilze the zinc. The results of the present study showed the diazotrophic bacteria associated to both wild and cultivated rice and it having variety of plant growth promoting substances in considerable amounts apart from diazotrophy.
Keywords
Rice, Diazotrophs, Oryza Indica, Oryza Sativa, Pgpr
Subscription
Login to verify subscription
User
Font Size
Information
- Bagnasco, P., De La Fuente, L., Gualtieri, G., Noya, F. and Anas, A. (1998). Fluorescent Pseudomonas spp. as biocontrol agents against forage legume ischolar_main pathogenic fungi. Soil Biol. Biochem., 30: 1317-1322.
- Beneduzi, A., Peres, D., Vargas, L.K., Bodanese-Zanettini, M.H. and Passaglia, L.M.P. (2008). Evaluation of genetic diversity and plant growth promoting activities of nitrogen-fixing Bacilli isolated from rice fields in South Brazil. Appl. Soil Ecol., 39: 311-320.
- Borrow, A., Brian, P.W., Chester, V.E., Curtis, P.J., Hemming, H.G., Henehan, C., Jeffreys, E.G., Lloyd, P.B., Nixon, I.S., Norris, G.L.F. and Radley, M. (1955). Gibberellic acid, a metabolic product of the fungus Gibberella fujikuroi: some observations on its production and isolation. J. Sci. Food agric., 6: 340-348.
- Bunt, J.S. and Rovira, A.D. (1955). Microbiological studies of some sub-antartic soils. J. Soil. Sci., 6:119-128.
- Burris, R.H. (1972). Nitrogen fixation - assay methods and techniques. In: Methods in enzymology. (Eds.) Colowick, S.P. and N.O. Kaplan. Academic press, NEW YORK (U.S.A.), pp. 415- 431.
- Burris, R.H. and Wilson, P.W. (1972). Methods for measurement of nitrogen fixation. In: Methods in enzymology. (Eds.) Colowick, S.P. and N.O. Kaplan. Academic press, NEW YORK (U.S.A.). pp. 355-367.
- Cappuccino, J.C. and Sherman, N. (1992). Negative staining. In:Microbiology: A laboratory manual, (Eds.). Cappuccino, J.C., N. Sherman and C. Benjamin. Redwood City, pp.125-179.
- Chandramohan, D. and Mahadevan, A. (1968). Indole acetic acid metabolism in soils. Curr. Sci., 37: 112-113.
- Dubey, R.C. and Maheshwari, D.K. (2004). Practical microbiology. S. Chand and company Ltd., NEW DELHI (INDIA).
- Fujii, T., Huang, Y.D., Higashitani, A., Nishimura, Y., Iyama, S., Hirota, Y., Yoneyama, Y. and Dixon, R.A. (1987). Effect of inoculation with Klebsiella oxytoca and Enterobacter cloacea on dinitrogen fixation by rice-bacteria associations. Plant Soil, 103: 221-226.
- Hossain, M. and Fischer K.S. (1995). Rice research for food security and sustainable agricultural development in Asia: Achievements and future challenges. Geo. J., 35: 286295.
- Humphries, E.C. (1956). Mineral composition and ash analysis. In: Modern methods of plant analysis. (Eds.) Peach, K. and M.V. Trecy. Springer Verlag, BERLIN (GERMANY). pp. 468-502.
- Katznelson, H. and Bose, B. (1959). Metabolic activity and phosphate-dissolving capability of bacterial isolates from wheat ischolar_mains, rhizosphere and non-rhizosphere soil. Canadian J. Microbiol., 5: 7985.
- Kirchhof, G., Reis, V.M., Baldani, J.I., Eckert, B., Dbereiner, J. and Hartmann, A. (1997). Occurrence, physiological and molecular analysis of endophytic diazotrophic bacteria in gramineous energy plants. Plant Soil., 194: 4555.
- Ladha, J.K., Barraquio, W.L. and Watanabe, I. (1983) Isolation and identification of nitrogen-fixing Enterobacter cloacae and Klebsiella panticola associated with rice plants. Canadian J. Microbial., 29: 1301-1308.
- Lowry, O.H., Rosebrough, J.J., Farr, A.L. and R.L. Randall. (1951). Protein measurements with folin phenol reagent. J. Biol. Chem., 193: 265-273.
- Lu, J.J. and Chang, T. (1980). Rice in its temporal and spatial perspectives. In: Rice:Production and utilization, Ed. B.S. Luh, p. 1-74, Avi Publishing, Westport, NEW ZEALAND (U.S.A.).
- Mortenson, L.E. (1951). A simple method for measuring nitrogen fixation by cell-free enzyme preparations of Clostridium pasteurianum. Analytical Biochem., 2(3): 216-220.
- Park, M., Kim, C., Yang, J., Lee, H., Shin, W., Kim, S. and Sa, T.M. (2005). Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea. Microbiol. Res., 160:127133.
- Piao, Z., Cui, Z., Yin, B., Hu, J., Zhou, C., Xie, G., Su, B. and Yin, S. (2005). Changes in acetylene reduction activities and effects of inoculated rhizosphere nitrogen-fixing bacteria on rice. Biol Fertil Soils., 41: 371378.
- Pramer, D. and Schmidt, E.L. (1966). Experimental soil microbiology. Burgess Publ. Co., Minn., Minneopolis. 107 pp.
- Reis, V.M., Olivares, FI. and Dobereiner, J. (1994) Improved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat. World J. Microbiol. Biotechnol., 10: 101104.
- Roger, P.A. and Ladha, J.K. (1992). Biological N2 fixation in wetland rice fields: Estimation and contribution to nitrogen balance. Plant Soil, 141: 4155.
- Sato, Y.I. (1994). Ecological-genetic studies on wild and cultivated rice in tropical Asia. Tropics, 3: 189-245.
- Sgroy, V., Cassan, F., Masciarelli, M.O., Papa, F.D., Lagares, A. and Luna, V. (2009). Isolation and characterization of endophytic plant growth promoting or stress-homeostasis regulating bacteria associated to the halophyte Prosopis strombulifera. Appl. Microbiol. Biotechnol., 7: 341-346.
- You, C. and Zhou, F. (1989). Non-nodular endorhizospheric nitrogen fixation in wetland rice. Canadian J. Microbiol., 35: 403-408
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 320
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 0