Asian Journal of Bio Science

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Induced Resistance and Phenolic Acid Accumulation in Biological Control of Chickpea Wilt by Pseudomonas fluorescens


Affiliations
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India
     

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HPLC analysis of chickpea (Cicer arietinum) plant at various wilt disease developmental stages challenged with Fusarium oxysporum f.sp ciceri indicates the plants contain high amount of phenolics when the seed was treated with Pseudomonas fluorescens local isolates Pf-3. This biocontrol bacterial isolate induced the synthesis of specific phenolic acids like salicylic acid, chlorogenic acid etc. with varied amounts at different disease development stages of wilt in chickpea. Vannilic, chlorogenic and hydroquinone were also found higher in plants treated with Fusarium oxysporum f.sp. ciceri.All the above mentioned phenolics was found higher amount in resistant variety than susceptible variety. The induction of antifungal phenolic acids in chickpea plant in the present investigation due to the application of the local isolate Pf-3 provides a biochemical basis of induced resistance in chickpea against wilt disease.

Keywords

Chickpea, Fusarium oxysporum F.sp. Ciceri, HPLC, Phenolics, Pseudomonas fluorescens, Salicylic Acid
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  • Lavania, M., Chauhan, P.S., Chauhan, S.V.S., Singh, H.B. and Nautiyal, C.S. (2006). Induction of plant defense enzymes and phenolics by treatment with plant growthpromoting rhizobacteria Serratia marcescens NBRI1213. Curr. Microbiol., 52: 363-368.

  • Malamy, J., Carr, J.P., Klessing, D.F. and Raskin, I. (1990). Salicylic acid: a likely endogenous signal in the resistance response of tobacco to tobacco mosaic virus. Science, 250: 10021004.

  • Matern, U. and Kneusel, R.E. (1988). Phenolic compounds in plant disease resistance. Phytoparasitica, 16: 158-170.

  • Metraux, J.P., Signer, H., Ryals, J., Ward, E., Wyss-Benz, M., Gaudin, J., Raschdorf, K., Schmid, E., Blum, W. and Inveraadi, B. (1990). Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. Science, 250: 10041006.

  • Mohamed, C., Arfaoui, A. and Rhaiem, A. (2007). Phenolic compounds and their role in bio-control and resistance of chickpea to fungal pathogenic attacks. Tunisian J. Pl. Pro., 2(1): 7-22.

  • Raskar, S.T., Kale, A.A. and Munjal, S.V. (2005). Identification of pathogenesis related proteins involved in Fusarium wilt resistance in chickpea cultivars. 4th international food legumes research conference, October 18-22, New delhi. IFLRC-Abstract. A-493. 294.pp.

  • Reddy, B.P., Reddy, K.R.N., Rao, M.S. and Rao, K.S. (2008). Efficacy of antimicrobial metabolites of Pseudomonas fluorescens against rice fungal pathogens. Curr. Tre. Biotech. & Pharma., 2: 178-182.

  • Ribnicky, D.M., Shulaev, V. and Ruskin, I. (1998). Intermediates of salicylic acid biosynthesis in tobacco. Plant Physiol., 118: 565572.

  • Sarma, B.K. and Singh, U.P. (2003). Ferulic acid may prevent infection in Cicer arietinum by Sclerotium rolfsii. World J. Microbiol. Biotech., 19: 123-127.

  • Sharma, B.K., Singh, D.P., Mehta, S., Singh, H.B. and Singh, U.P. (2002). Plant growth promoting rhizobacteria-elicited alterations in phenolic profile of chickpea (Cicer arietinum L.) infected by Sclerotium rolfsii. J. Phytopathol., 150: 277-282.

  • Simon, A. and Ridge, E.H. (1974). The use of ampicillin in a simplified selective medium for the isolation of fluorescent Pseudomonas. J. Appl. Bacteriol., 37: 459-460.

  • Singh, R.B., Singh, H.N., Singh, P. and Kaur, J. (2001). A comparision of different substrates for the mass production of Trichoderma. Ann. Pl. Prot. Sci., 9: 248-253.

  • Singh, U.P., Prithiviraj, B., Singh, K.P. and Sarma, B.K. (2000). Control of powdery mildew (Erysiphe pisi) of pea (Pisum sativum) by combined application of plant growth-promoting rhizobacteria and NeemazalTM. J. Plant Dis. Prot., 107: 59-66.

  • Singh, U.P., Sarma, B.K., and Singh, D.P. (2003). Effect of plant growth promoting rhizobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (Cicer arietinum). Curr. Microbiol., 46: 131-140.

  • Stevenson, P.C. and Veitch, N.C. (1998). A-2 arylbenzofuran from ischolar_mains of Cicer bijugam associated with Fusarium wilt. Phytochem., 48:947-951.

  • Subramanian, C.V. (1954). Studies on south India fusarium III. Fusarium isolated from some crop plants. J. Madras. Univ., 24: 21-46.

  • Vlassak, K., Van holm, L., Duchateau, L., Vanderleyden, J. and De Mot, R. (1992). Isolation and characterization of fluorescent Pseudomonas associated with the ischolar_mains of rice and banana grown in Sri Lanka. Pl. Soil, 145: 51-63.

  • Zarrin, F., Saleemi, M., Zia, M., Sultan, T., Aslam, M., Rehman, R. and Chaudhary, M.F. (2009). Antifungal activity of plant growthpromoting rhizobacteria isolates against Rhizoctonia solani in wheat. Afri. J. Biotech., 8(2): 219225.


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  • Induced Resistance and Phenolic Acid Accumulation in Biological Control of Chickpea Wilt by Pseudomonas fluorescens

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Authors

U. K. Kandoliya
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India
D. N. Vakharia
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India

Abstract


HPLC analysis of chickpea (Cicer arietinum) plant at various wilt disease developmental stages challenged with Fusarium oxysporum f.sp ciceri indicates the plants contain high amount of phenolics when the seed was treated with Pseudomonas fluorescens local isolates Pf-3. This biocontrol bacterial isolate induced the synthesis of specific phenolic acids like salicylic acid, chlorogenic acid etc. with varied amounts at different disease development stages of wilt in chickpea. Vannilic, chlorogenic and hydroquinone were also found higher in plants treated with Fusarium oxysporum f.sp. ciceri.All the above mentioned phenolics was found higher amount in resistant variety than susceptible variety. The induction of antifungal phenolic acids in chickpea plant in the present investigation due to the application of the local isolate Pf-3 provides a biochemical basis of induced resistance in chickpea against wilt disease.

Keywords


Chickpea, Fusarium oxysporum F.sp. Ciceri, HPLC, Phenolics, Pseudomonas fluorescens, Salicylic Acid

References