Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Molecular Characterization and Identification of an Alkalophilic Bacterial Strain Isolated from a Local Hotspring Atri, Khurda District, Odisha, India


Affiliations
1 Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneshwar, Orissa, India
2 First Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyanga, Bhubaneshwar, Orissa, India
     

   Subscribe/Renew Journal


The present study was conducted to isolate, identify, characterize and to determine the enzymatic activities of the thermophiles from Atri hot spring of odisha. The optimal temperature for growth of these isolates was 55 C and the optimal pH was 8. These bacterial cells were Gram positive rods and endospore forming. All the strains were amylase, catalase and oxidase positive but gelatinase and caseinase negative but isolate A1 showed the best amylase producing. The phenotypic characterization of those isolates was confirmed by genotypic method using 16S rDNA sequence analysis. Maximal homology of isolate A1 to genus Geobacillus was observed. Isolate A1 showed 96 per cent homology with Geobacillus sp. WCH 70 (Accession no. NC 012793.1). Therefore, 16S rDNA gene sequence analysis can be considered as a valuable genotypic tool for the identification and characterization of this thermophilic bacterium at genus level. Moreover, enzymatic products of these isolates could receive considerable attention due to their potential applications in biotechnology.

Keywords

Thermophiles, Enzymes, Hot Spring, 16S rDNA.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Al-Batayneh, K.M., Jacob, J.H. and Hussein, E.I. (2011). Isolation and molecular identification of new thermophilic bacterial strains of Geobacillus pallidus and Anoxybacillus flavithermus. Internat. J. Integ. Biol., 11(1): 39-43.

  • Bae, S.S., Lee, J.H. and Kim, S.J. (2005). Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Internat. J. Syst. Evol. Microbiol., 55: 1211-1215.

  • Baker, G., Gaffar, S., Cowan, D.A. and Suharto, A.R. (2001). Bacterial community analysis of Indonesian hotsprings. FEMS Microbiol. Lett., 200: 103-109.

  • Bergquist, P.C. and Morgan, H.W. (1992). The molecular genetics and biotechnological application of enzyme from extremely thermophilic eubacteria. In: Herbert RA, Sharp RJ (eds)Molecular biology and biotechnology of extremophiles. Chapman and Hall, New York, pp. 44-45.

  • Bertoldo, C. and Antranikian, G. (2002). Starch-hydrolyzing enzymes from thermophilic archea and bacteria. Curr. Opin. Chem. Biol., 6: 151-160.

  • Lama, L., Calandrelli, V., Gambacorta, A. and Nicolaus, B. (2004). Purification and characterization of thermostable xylanase and betaxylosidase by the thermophilic bacteriumBacillus thermantarcticus. Res. Microbiol., 155: 283-289.

  • Lu, Y., Chen, B., Liu, X., Ji, X., Wei, Y. and Lin, L. ( 2009). Isolation and identification of seven thermophilic and anaerobic bacteria from hot springs in Tengchong Rehai.Wei Sheng Wu Xue Bao., 49(9): 1234-1239.

  • Maugeri, T.L., Gugliandolo, C., Caccamo, D. and Stackebrandt, E. (2001). A polyphasic taxonomic study of thermophilic bacilli from shallow, marine vents. Syst. Appl. Microbiol., 24: 572-587.

  • Meintanis, C., Chalkou, K.I., Kormas, K.A. and Karagouni, A.D. (2006). Biodegradation of crude oil by thermophilic bacteria isolated from a volcano island. Biodegradation, 17: 105-111.

  • Narayan, V.V., Hatha, M.A., Morgan, H.W. and Rao, D. (2008). Isolation and characterization of aerobic thermophilic bacteria from Savusavu hot spring in Fiji.Microbes Environ., 23(4): 350-352.

  • Nazina, T.N., Tourova, T.P., Poltaraus, A.B., Novikova, E.V., Grigoryan, A.A., Ivanova, A.E., Lysenko, A.M., Petrunyaka, V.V., Osipov, G.A., Belyaev, S.S. and Ivanov, M.V. (2001). Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov., and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Internat. J. Syst. Evon. Microbiol., 51: 433-446.

  • Rahman, T.J., Marchant, R. and Banat, I.M. (2004). Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments. Biochem. Soc. Trans., 32: 209-213.

  • Rainey, F.A., Fritze, D. and Stackebrandt, E. (1994). The phylogenetic diversity of thermophilic members of the genus Bacillus as revealed by 16S rDNA analysis. FEMS Microbiol. Lett., 115: 205-212.

  • Rath, C.C. and Subramanyam, V.R. (2000). Enhanced protease and beta-Lactamase activity by immobilization of a thermophilic bacillus sps. isolated from a local hotspring in Orissa, India. Proceeding of national conference in recent trends in biotechnology.

  • Schallmey, M., Singh, A. and Ward, O.P. (2004). Developments in the use of Bacillus species for industrial production. Can. J. Microbiol., 50: 1-17.

  • Sharp, R.J., Riley, P.W. and White, D. (1992). Heterotrophic thermophilic bacilli. In: Kristjansson JK (ed) Thermophilic bacteria. CRC Press, Boca Raton, pp. 19-50.


Abstract Views: 472

PDF Views: 0




  • Molecular Characterization and Identification of an Alkalophilic Bacterial Strain Isolated from a Local Hotspring Atri, Khurda District, Odisha, India

Abstract Views: 472  |  PDF Views: 0

Authors

S. P. Nayak
Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneshwar, Orissa, India
P. Ray
Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneshwar, Orissa, India
P. K. Sahoo
First Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyanga, Bhubaneshwar, Orissa, India

Abstract


The present study was conducted to isolate, identify, characterize and to determine the enzymatic activities of the thermophiles from Atri hot spring of odisha. The optimal temperature for growth of these isolates was 55 C and the optimal pH was 8. These bacterial cells were Gram positive rods and endospore forming. All the strains were amylase, catalase and oxidase positive but gelatinase and caseinase negative but isolate A1 showed the best amylase producing. The phenotypic characterization of those isolates was confirmed by genotypic method using 16S rDNA sequence analysis. Maximal homology of isolate A1 to genus Geobacillus was observed. Isolate A1 showed 96 per cent homology with Geobacillus sp. WCH 70 (Accession no. NC 012793.1). Therefore, 16S rDNA gene sequence analysis can be considered as a valuable genotypic tool for the identification and characterization of this thermophilic bacterium at genus level. Moreover, enzymatic products of these isolates could receive considerable attention due to their potential applications in biotechnology.

Keywords


Thermophiles, Enzymes, Hot Spring, 16S rDNA.

References