Asian Journal of Bio Science

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Evaluation of Local Bacillus thuringiensis from the Soils of Westernghats, Karnataka and their Biocontrol Potential against White Grub, Holotrichia serrata (F.) (coleoptera) and House Fly, Musca domestica L. (Diptera)


Affiliations
1 Department of Biotechnology, Bangalore University, Bengaluru, Karnataka, India
2 Department of Entomology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India
     

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The Western Ghats of India is one of the world's "biodiversity hotspots" that runs along the western part of South India through four states including Karnataka. As a result, Western Ghats are expected to yield high diversity of any taxon. With a view to understand this aspect study on the diversity of Bacillus spp. in the soils of Western Ghats was conducted. A total of 292 Bacillus isolates were identified as Bacillus thuringiensis which were recovered from 35 soil samples collected from different habitats of Western Ghats of Karnataka. Soils of different habitats varied tremendously in the natural load of Bacillus CFUs. Lowest CFU load was observed in soil W15 (2.6 x 106 ) whereas the soils W13, W20, W24, W29 (8.1 x 106) yielded the highest number of Bacillus CFUs/g of soil, with an overall mean of 6.07 x 106 CFUs per g of soil. On an average, 8.34 ( 1.95) colonies were picked from each soil sample. These colonies were subjected to standard biochemical tests to identify the B. thuringiensis colonies. On an average, 5.6 (67.12 %) of the picked colonies per soil sample were observed to be B. thuringiensis colonies. Tests of activity of these isolates against a species of white grub, Holotrichia serrata (F.) and a fly pest, Musca domestica revealed 14 isolates to be active against H. serrata and 10 against M. domestica, with three of these against both the species. The study thus demonstrated that there is potential for the use of these isolates in pest management.

Keywords

Bacillus Thuringiensis, Endospores, Toxicity, Holotrichia Serrata, Musca Domestica
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  • Evaluation of Local Bacillus thuringiensis from the Soils of Westernghats, Karnataka and their Biocontrol Potential against White Grub, Holotrichia serrata (F.) (coleoptera) and House Fly, Musca domestica L. (Diptera)

Abstract Views: 282  |  PDF Views: 2

Authors

Manju Kumari
Department of Biotechnology, Bangalore University, Bengaluru, Karnataka, India
D. Manjulakumari
Department of Biotechnology, Bangalore University, Bengaluru, Karnataka, India
A. R. V. Kumar
Department of Entomology, University of Agricultural Sciences, G.K.V.K., Bengaluru, Karnataka, India

Abstract


The Western Ghats of India is one of the world's "biodiversity hotspots" that runs along the western part of South India through four states including Karnataka. As a result, Western Ghats are expected to yield high diversity of any taxon. With a view to understand this aspect study on the diversity of Bacillus spp. in the soils of Western Ghats was conducted. A total of 292 Bacillus isolates were identified as Bacillus thuringiensis which were recovered from 35 soil samples collected from different habitats of Western Ghats of Karnataka. Soils of different habitats varied tremendously in the natural load of Bacillus CFUs. Lowest CFU load was observed in soil W15 (2.6 x 106 ) whereas the soils W13, W20, W24, W29 (8.1 x 106) yielded the highest number of Bacillus CFUs/g of soil, with an overall mean of 6.07 x 106 CFUs per g of soil. On an average, 8.34 ( 1.95) colonies were picked from each soil sample. These colonies were subjected to standard biochemical tests to identify the B. thuringiensis colonies. On an average, 5.6 (67.12 %) of the picked colonies per soil sample were observed to be B. thuringiensis colonies. Tests of activity of these isolates against a species of white grub, Holotrichia serrata (F.) and a fly pest, Musca domestica revealed 14 isolates to be active against H. serrata and 10 against M. domestica, with three of these against both the species. The study thus demonstrated that there is potential for the use of these isolates in pest management.

Keywords


Bacillus Thuringiensis, Endospores, Toxicity, Holotrichia Serrata, Musca Domestica

References