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Effect of Different Nitrogen Concentrations on the Biomass and Biochemical Consituents of Spirulina platensis [Geitler]


Affiliations
1 Department of Agricultural Microbiology, Agricultural College and Research Insitute, Madurai, T.N., India
     

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Nitrogen is one of the primary requirements of growth media for any cell. Absence of nitrogen or starvation condition is considered as stress by the organisms. Increasing the nitrogen concentration upto 0.04 M significantly increased the biomass, protein, phycocyanin and lipid content of Spirulina, while total carotenoids and ?-carotene content were reduced compared to control. In contrast, the biomass, protein, phycocyanin and lipid content were reduced in the control indicating that nitrogen requirement for synthesis of aminoacids, which make up protein and other cellular components such as phycocyanin. The accumulation of carotenoids under nitrogen starvation may be due to production of a large amount of acetyl-CoA, which serves as a precursor for synthesis of carotenoids.

Keywords

Spirulina Platensis, Nitrogen, Biomass, Pigment, Lipids
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  • Bhattacharya, S. and Shivaprakash, M.K. (2005). Evaluation of three Spirulina species grown under similar conditions for their growth and biochemicals. J. Sci. Food Agric., 85: 333336.
  • Bligh, E.G. and Dyer, W.J. (1959). A rapid method for total lipid extraction and purification.Can.J.Biochem.Physiol., 37: 911-917.
  • Boussiba, S. and Richmond, A. (1979). Isolation and characterization of phycocyanin from the blue-green alga Spirulina platensis.Arch. Microbiol., 120: 155-159.
  • El-Baz, F.K., Aboul-Enein, A.M., El-Baroty, G.S., Youssef, A.M. and Abd El-Baky, H. (2002). AnticarcinogenicAnticarcinogenic activity of algal extracts.J.Med. Sci., 2: 243-251. Estrada J.E., Bescos P. and Villar Del Fresno A.M.2001.Antioxidant activity of different fractions of Spirulinaplatensis protean extract.Il Farmaco, 56,497-500.
  • Filali, R., Lasseur, C. and Christophe, G. (1997). Nitrogen sources for growth of the cyanobacteriumSpirulina. Sixth European Symposium on Space Environmental Control Systems, held in Noordwijk, The Netherlands, 20-22 May.
  • Hanaa, H. and Abd El-Baky (2003). Over production of phycocyanin pigment in blue green alga Spirulina sp. and its inhibitory effect on growth of carcinoma cells. J. Med. Sci., 3: 314-324.
  • Kim, C.J. and Jung, Y.H. (2007). Factors indicating culture status during cultivation of Spirulina (Arthospira) platensis. J. Microbiol., 45(2): 122-127.
  • Lowry, O.H., Rosebrough, N.L., Farr, A.L. and Radall, R.J. (1951). Protein measurement with the folin-phenol reagent. J. Bio. Chem., 193: 265-275.
  • Moller, A.P, Biard, C., Blount, J.D., Houston, D.C., Ninni, P., Saino, N. and Surai, P.F. (2000). Carotenoid-dependent signals: indicators of foragingefficiency, immune-competence or detoxification ability? Avian Poult. Biol. Rev., 11: 137-159.
  • Pisal, D.S. and Lele, S.S. (2005). Carotenoid production from microalga, Dunaliellasalina, Indian J. Biotech., 4: 476-483.
  • Rafiqul, I.M., Hassan, A., Sulebele, G., Orosco, C.A., Roustaian, P. and Jalal, C.A. (2003). Salt stress culture of blue green algae Spirulina fusiformis. Pak. J Biol. Sci. 6(7): 648-650.
  • Vonshak, A. (1997). Spirulina platensis (Arthrospira).Physiology, cell biology and biotechnology. Taylor and Francis, LONDON, UNITED KINGDOM.
  • Zarrouk, C. (1966). Contribution ltudedunecyanophyce. Influence de divers facteurs physiques etchimiquessur la croissance et la photosynthse de Spirulina maxima. Ph.D. Thesis, Universit de Paris, PARIS.

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  • Effect of Different Nitrogen Concentrations on the Biomass and Biochemical Consituents of Spirulina platensis [Geitler]

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Authors

Sujatha Kand
Department of Agricultural Microbiology, Agricultural College and Research Insitute, Madurai, T.N., India
P. Nagarajan
Department of Agricultural Microbiology, Agricultural College and Research Insitute, Madurai, T.N., India

Abstract


Nitrogen is one of the primary requirements of growth media for any cell. Absence of nitrogen or starvation condition is considered as stress by the organisms. Increasing the nitrogen concentration upto 0.04 M significantly increased the biomass, protein, phycocyanin and lipid content of Spirulina, while total carotenoids and ?-carotene content were reduced compared to control. In contrast, the biomass, protein, phycocyanin and lipid content were reduced in the control indicating that nitrogen requirement for synthesis of aminoacids, which make up protein and other cellular components such as phycocyanin. The accumulation of carotenoids under nitrogen starvation may be due to production of a large amount of acetyl-CoA, which serves as a precursor for synthesis of carotenoids.

Keywords


Spirulina Platensis, Nitrogen, Biomass, Pigment, Lipids

References