Asian Journal of Bio Science

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Role of Fluorescent Pseudomonas in Reduction of the Use of Chemical Pesticides and Fungicides in Normal and Replant Sites of Apple and Pear


Affiliations
1 Department of Basic Science, Dr. Y.S. Parmar University of Horticulture and forestry, Nauni, Solan, H.P., India
     

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Phytopathogenic microbes including few fungi and bacteria have an immense effect on the agricultural productivity, greatly reducing crop yield and sometimes causing total crop loss. However, the fungicides being used have the negative effects on the environment and human health. In this context, biological control of plant diseases is gaining attention due to increased pollution concerns and is an alternative method to control pathogenic fungi. So, the current trends in agriculture are focused on reduction in the use of chemical pesticides and fungicides, compelling the search for alternatives that enhance environmental quality. Effective option is to employ the pathogen's natural enemies as biocontrol agents, which is less destructive and environmental friendly. PGPR are a group of bacteria that actively colonize plant ischolar_mains and increase plant growth and yield. Among various biocontrol agents, Pseudomonas sp., equipped with multiple mechanisms of biocontrol of phytopathogens is being used widely. Biological control of plant pathogens is through the production of antibiotics, lytic enzyme, siderophore and HCN production. In the present study, the Pseudomonas spp. were isolated from the rhizosphere of apple and pear and screened for their biocontrol properties such as antifungal activity, siderophore production and HCN production. Out of 30 isolates, 15 isolates showed antifungal activity against Fusarium sp. and Alternaria spp. Maximum isolates showed siderophore production. All isolates were found to be positive for ammonia and HCN production. Taken together, results suggest that Pseudomonas spp. is an efficient approach to replace agrochemicals and pesticides, thereby reducing their negative effects on the environment.

Keywords

Apple, Pear, Pseudomonas, Siderophore, Antifungal Activity, Proteolytic Activity
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  • Ahmad, F., Ahmad, I. and Khan, M.S. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbial Res., 163(2): 173-181.

  • Bakker, A.W. and Schippers, B. (1987). Microbial cyanide production in the rhizosphere to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biol. & Biochem., 19: 451-457.

  • Cattelan, M.E., Hartel, P.G. and Fuhrmann, J.J. (1999). Screening of plant growth promoting rhizobacteria to promote early soybean growth. Soil Sci. Soc. America., 63: 1670-1680.

  • Chaiharn, M., Chunhaleuchanon, S., Kozo, A. and Lumyong, S. (2008). Screening of rhizobacteria for their plant growth promoting activities. KMITL Sci. Tech. J., 8(1): 18-23.

  • Chincholkar, S.B., Chaudhari, B.L, Rane, M.R. and Sarode, P.D. (2007). Fungal phytopathogen suppression using siderophoregenic bioinoculants. In: Biological control of plant diseases: current concepts. Chincholkar S B and Mukerji K G (eds.). USA: Haworth Press. pp. 401-417.

  • Gerhardson, B. (2002). Biological substitutes for pesticides. Trends Biotechnol., 20: 338-343.

  • Gomez, K.A. and Gomez, A.A. (1984). Statistical procedure for agricultural research New York. John Willey 680 pp.

  • Gurinot, M.L. (1994). Microbial iron transport. Annu. Rev. Microbiology. London: academic Press.

  • Haas, D., Keel, C. and Reimmann, C. (2002). Signal transduction in plant-beneficial rhizobacteria with biocontrol properties. Antonie Leeuwenhoek, 81: 385-395.

  • Heydari, S., Moghadam, P.R. and Arab, S.M. (2008). Hydrogen cyanide production ability by Pseudomonas fluorescens bacteria and their inhibition potential on weed. In: Proceedings Competition for Resources in a Changing World: New Drive for Rural Development. Tropentag, Hohenheim.

  • Hillel, D. (2005). In: Encyclopedia of soils in the environment, 1:103-115.

  • Huang, Z., Bonsall, R.F., Mavrodi, D.V., Weller, D.M. and Thomashow, L.S. (2004). Transformation of Pseudomonas fluorescens with genes for biosynthesis of phenazine-1-carboxylic acid improves biocontrol of Rhizoctonia ischolar_main rot and in situ antibiotic production. FEMS Microbiol. Ecol., 49: 243-251.

  • Kaur, M., Gupta, M., Tripathi, K.K. and Gupta, K.G. (1989). Lytic effect of Pseudomonas aeruginosa elastase on Gram positive and Gram negative bacteria: Zentra Blatt Bakt. Internat. J. Microbiol., 34: 855-859.

  • Kilic-Ekici, O. and Yuen, G.Y. (2004). Comparison of strains of Lysobacter enzymogenes and PGPR for induction of resistance against Bipolaris sorokiniana in tall fescue. Biol. Control, 30: 446-455.

  • Kloepper, J.W., Rodriguez-Ubana, R., Zehnder, G.W., Murphy, J.F., Sikora, E. and Fernandez, C. (1999). Plant ischolar_main-bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases. Austral. Plant Pathol., 28: 21-26.

  • Lata and Saxena, A.K. (2003). Characterization of plant growth promoting rhizobacteria. In: Training manual on biofertilizer technology (eds.) A K Saxena. IARI. Delhi.pp.24-25.

  • Mark, G.L, Morrissey, J.P., Higgins, P. and OGara, F. (2006). Molecular-based strategies to exploit Pseudomonas biocontrol strains for environmental biotechnology applications. FEMSMicrobiol. Ecol., 56: 167-77.

  • Mayak, S., Tirosh, T. and Glick, B.R. (2004). Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol. Biochem., 42: 565-572.

  • Meyer, J.M. (2000). Pyoverdins: Pigments siderophores and potential taxonomic markers of fluorescent Pseudomonas species. Archaea Microbiol., 174: 135-142.

  • Meyer, J.M. and Abdallah, M.A. (1978). The fluorescent pigment ofPseudomonas fluorescens: biosynthesis, purification and physicochemical properties. J. General Microbiol., 107: 319-328.

  • Nair, J.R, Singh, G. and Sekar, V. (2002). Isolation and characterization of a novel Bacillus strain from coffee phyllosphere showing antifungal activity. J. Appl. Microbiol., 93: 772-780.

  • Nelson, L.M. (2004). Plant growth promoting rhizobacteria (PGPR): Prospects for new inoculants. Online. Crop Management doi:10.1094/CM- 2004-0301-05-RV.

  • Pandey, A., Trivedi, P., Kumar, B. and Palni, L.M. (2006). Characterization of a phosphate solubilizing and antagonistic strain ofPseudomonas putida (BO) isolated from a subalpine location in the Indian Central Himalaya. Curr. Microbiol., 53(2): 102-107.

  • Rakh, R.R, Raut, L.S, Dalvi, S.M. and Manwar, A.V. (2011). Biological control of Sclerotium rolfsii, causing stem rot of groundnut by Pseudomonas cf. monteilii 9. Recent Res. Sci. & Technol., 3(3): 26-34.

  • Ramyasmruthi, S., Pallavi, O., Pallavi, S., Tilak, K. and Srividya, S. (2012). Chitinolytic and secondary metabolite producing Pseudomonas fluorescens isolated from Solanaceae rhizosphere effective against broad spectrum fungal phytopathogens. Asian J. Plant Sci. & Res., 2(1): 16-24.

  • Sarode, P.D., Rane, M.R., Chaudhari, B.L. and Chincholkar, S.B. (2009). Siderophoregenic Acinetobacter calcoaceticus isolated from wheat rhizosphere with strong PGPR activity. Malaysian J. Microbiol., 5(1): 6-12.

  • Schwyan, B. and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytic Biochem. 28(8): 751-759.

  • Sivaramaiah, N., Malik, D.K. and Sindhu, S.S. (2007). Improvement in symbiotic efficiency of chickpea (Cicer arietinum) by coinoculation of Bacillus strains with Mesorhizobium sp Cicer. Indian J. Microbiol., 47: 51-56.

  • Tilak, K.V.B.R., Singh, G. and Mukerji, K.G. (1999). Mukerji K G (eds). Biocontrol- Plant growth promoting rhizobacteria: Mechanisms of action. Biotechnological Approaches Biocontrol Plant Pathogens, 89(1): 136-150.

  • Vansuyt, G., Robin, A., Briat, J.F., Curie, C. and Lemanceau, P. (2007). Iron acquisition from Fe-pyoverdine by Arabidopsis thaliana. Molecular Plant Microbe Interactions, 20: 441-447.

  • Villegas, J. and Fortin, J.A. (2002). Phosphorous solubilization and pH changes as a result of the interactions between soil bacteria and arbuscular mycorrhizae fungi on a medium containing NO3 as nitrogen source. Canadian J. Bot., 80(5): 571-576.

  • Vincent, J.M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 150: 850.

  • Welbaum, G., A.V., Sturz, Dong, Z. and Nowak, J. (2004). Fertilizing soil microorganisms to improve productivity of agroecosystems. Crit. Rev. Plant Sci., 23: 175-193.


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  • Role of Fluorescent Pseudomonas in Reduction of the Use of Chemical Pesticides and Fungicides in Normal and Replant Sites of Apple and Pear

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Authors

Deep Shikhathakur
Department of Basic Science, Dr. Y.S. Parmar University of Horticulture and forestry, Nauni, Solan, H.P., India
Mohinder Kaur
Department of Basic Science, Dr. Y.S. Parmar University of Horticulture and forestry, Nauni, Solan, H.P., India
Vineet Shyam
Department of Basic Science, Dr. Y.S. Parmar University of Horticulture and forestry, Nauni, Solan, H.P., India

Abstract


Phytopathogenic microbes including few fungi and bacteria have an immense effect on the agricultural productivity, greatly reducing crop yield and sometimes causing total crop loss. However, the fungicides being used have the negative effects on the environment and human health. In this context, biological control of plant diseases is gaining attention due to increased pollution concerns and is an alternative method to control pathogenic fungi. So, the current trends in agriculture are focused on reduction in the use of chemical pesticides and fungicides, compelling the search for alternatives that enhance environmental quality. Effective option is to employ the pathogen's natural enemies as biocontrol agents, which is less destructive and environmental friendly. PGPR are a group of bacteria that actively colonize plant ischolar_mains and increase plant growth and yield. Among various biocontrol agents, Pseudomonas sp., equipped with multiple mechanisms of biocontrol of phytopathogens is being used widely. Biological control of plant pathogens is through the production of antibiotics, lytic enzyme, siderophore and HCN production. In the present study, the Pseudomonas spp. were isolated from the rhizosphere of apple and pear and screened for their biocontrol properties such as antifungal activity, siderophore production and HCN production. Out of 30 isolates, 15 isolates showed antifungal activity against Fusarium sp. and Alternaria spp. Maximum isolates showed siderophore production. All isolates were found to be positive for ammonia and HCN production. Taken together, results suggest that Pseudomonas spp. is an efficient approach to replace agrochemicals and pesticides, thereby reducing their negative effects on the environment.

Keywords


Apple, Pear, Pseudomonas, Siderophore, Antifungal Activity, Proteolytic Activity

References