Asian Journal of Bio Science

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Antipathogenic Potentiality of Fluorescent Pseudomonads for the Management of Rice Sheath Blight Pathogen, Rhizoctonia solani


Affiliations
1 Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), India
     

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Sheath blight (ShB) of rice is an important soil-borne fungal disease caused by Rhizoctonia solani (Kuhn) causing up to 40 per cent of yield losses annually. The present investigation was aimed to study the effect of Fluorescent pseudomonads on sheath blight management in rice and impact on plant growth. Fifteen different pseudomonad isolates were evaluated for their antagonistic activity against Rhizoctonia solani isolates under in vitro condition. Per cent inhibition of mycelial growth of R. solani by pseudomonads ranged from 74-100. All the isolates showed antagonism against the pathogen. Five strains, 12, 20, 19, soy2 and soy6 were found potent with 87-100 per cent inhibition of mycelial growth. They were further evaluated in greenhouse as seed treatment, soil application, foliar spraying and combined (seed+soil+foliar application) treatment for sheath blight control. Fluorescent pseudomonad isolate 19 was found potent and promising as it reduced the disease to the maximum extent and stimulated plant and ischolar_main growth.

Keywords

Rice, Rhizoctonia solani, Sheath Blight, Fluorescent Pseudomonads, Biocontrol.
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  • Anitha, A. and Das, M.A. (2011). Activation of rice plant growth against Rhizoctonia solani using Pseudomonas fluorescens, Trichoderma and salicylic acid. Res. Biotechnol., 2 (4) : 7-12.

  • Chen, C.C. and Chu, C.L. (1973). Studies on the control of rice blast and sheath blight of rice with benlate. J.Taiwan Agric. Res. New., 11: 22.

  • Crowe, J.H., Crowe, L.M., Oliver, A.E., Tsvetkova, N., Wolkers, W. and Tablin, F. (2001). The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state. Cryobiol., 43 : 89–105.

  • Dev, V.P.S. and Mary, C.A. (1986). Sheath blight (ShB) Control. Intl. Rice Res. Newslr., 11 : 22

  • Frankenberger, W.T. Jr. and Arshad, M. (1995). Phytohormones in soil: Microbial production and function. New York, Marcel Dekker.

  • Gasoni, L., Cozzi, J., Kobayashi, K., Yossen, V., Zumelzu, G. and Babbit, S. (1998). Suppressive effect of antagonistic agents on Rhizoctonia isolates on lettuce and potato in Argentina field plots. In: Intl. Cong. Pl. Pathol., 7th, 9th-16th August. Argentina.

  • Gupta, C.D., Dubey, R.C., Kang, S.C. and Maheshwari, D.K. (2001). Antibiotic mediated necrotrophic effect of Psedomonas GRC2 against two fungal plant pathogens. Current Sci., 81: 91-94.

  • Gupta, C.P., Dubey, R.C. and Maheshwari, D.K. (2002). Plant growth enhancement and suppression of Macrophomina phaseolina causing charcoal rot of peanut by fluorescent pseudomonads. Biol. Fertil. Soils, 35 : 399-405.

  • IRRI (1980). International Rice Research Institute, Annual Report for 1979. Los Banos, Philippines, pp: 171-182.

  • Kazempour, M.N. (2004). Biological control of R. solani, the causal agent of rice sheath blight by antagonistics bacteria in greenhouse and field condition. Pl. Pathol. J., 3 (2) : 88-96.

  • Keel, C. and Defago, G. (1997). Interactions between beneficial soil bacteria and ischolar_main pathogens : mechanisms and ecological impact. In : Multitrophic interactions in terrestrial system, Eds. Gange, A.C., Brown, V. K., Oxford Blackwell Science, pp. 27-47.

  • Lin, A., Lee, T.M. and Rern, J.C. (1994). Tricholin, a new antifungal agent from Trichoderma viride and its action in biological control of Rhizoctonia solani. J. Antibiotics, 47 : 799-805.

  • Ou, S.H. (1985). Rice diseases. Commonwealth Mycological Institute, Key, Surrey.

  • Park, J.L., Rand, R.E. and King, E.B. (1991). Biological control of pythium damping-off and phenomyces ischolar_main rot of peas by application of Pseudomonas cepacia or P. flourescens to seed. Plant Dis., 75 : 987-992.

  • Pathak, A., Sharma, A., Johsi, B.N. and Sharma, A.K. (2004). Pseudomonas strain GRP3 induces systemic resistance to sheath blight in rice, IRRN, 29 : 35-36.

  • Premlatha and Dath, A. (1990). Sheath blight disease of rice and its management. Associated Publishing Co., New Delhi, pp. 129.

  • Reddy, B.P., Reddy, K.R.N., Reddy, M.S., Kumar, Vijay Krishna K. and Sudini, H. (2010). Biological control of rice sheath blight disease by Pseudomonas fluorescens isolates. Int. J. Appl Bio. Pharma. Tech., 1 (2) : 666-669.

  • Rini, C.R. and Sulochana, K.K. (2007). Usefulness of Trichoderma and Pseudomonas against Rhizoctonia solani and Fusarium oxysporum infecting tomato. J. Tropi. Agric., 45 (1-2) : 21–28.

  • Sayyed, R.Z., Badgujar, M.D., Sonawane, H.M., Mhaske, M.M. and Chincholkar, S.B. (2005). Production of microbial iron chelators (siderophores) by fluorescent Pseudomonads, Indian J. Biotechnol., 4 : 484-490.

  • Thilagavathi, R., Kumar, Saravana D., Ragupathi, N. and Samiyappan, R. (2007). A combination of biocontrol agents improves the management of dry ischolar_main rot (Macrophomina phaseolina) in greengram. Phytopathol. Mediterr., 46: 157-167.

  • Vidhyasekaran, P. and Muthamilan, M. (1999). Evaluation of powder formulation of Pseudomonas yuorescens Pf1 for control of rice sheath blight. Biocontrol Sci. Technol., 9 (67) : 74.

  • Vincent, J.M. (1947). Distortion of fungal hyphae in presence of certain inhibitors. Nature, 159 : 850.

  • Wilson, H., Epton, H.A.S. and Sigee, D.C. (1992). Biological control of fire blight of Howthorn with fluorescent pseudomonas spp. under protected conditions. J. Phytopathol., 136 : 16-26.


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  • Antipathogenic Potentiality of Fluorescent Pseudomonads for the Management of Rice Sheath Blight Pathogen, Rhizoctonia solani

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Authors

Snehal J. Shinde
Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), India
S. K. Prashanthi
Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), India

Abstract


Sheath blight (ShB) of rice is an important soil-borne fungal disease caused by Rhizoctonia solani (Kuhn) causing up to 40 per cent of yield losses annually. The present investigation was aimed to study the effect of Fluorescent pseudomonads on sheath blight management in rice and impact on plant growth. Fifteen different pseudomonad isolates were evaluated for their antagonistic activity against Rhizoctonia solani isolates under in vitro condition. Per cent inhibition of mycelial growth of R. solani by pseudomonads ranged from 74-100. All the isolates showed antagonism against the pathogen. Five strains, 12, 20, 19, soy2 and soy6 were found potent with 87-100 per cent inhibition of mycelial growth. They were further evaluated in greenhouse as seed treatment, soil application, foliar spraying and combined (seed+soil+foliar application) treatment for sheath blight control. Fluorescent pseudomonad isolate 19 was found potent and promising as it reduced the disease to the maximum extent and stimulated plant and ischolar_main growth.

Keywords


Rice, Rhizoctonia solani, Sheath Blight, Fluorescent Pseudomonads, Biocontrol.

References