Asian Journal of Bio Science

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Bioefficacy of Different Species of Entomopathogenic Fungi Against White Grub, Leucopholis lepidophora (Blanchard) Infesting Sugarcane in Maharashtra


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1 Division of Entomology, Mahatma Phule Krishi Vidyapeeth, College of Agriculture, Kolhapur (M.S.), India
     

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The bioefficacy of three species of entomopathogenic fungi viz., Metarhizium anisopliae (Metsch.) Sorokin, Beauveria brongniartii (Sacc.) and Beauveria bassiana (Balsana) Vuillrmin, against white grub, Leucopholis lepidophora (Blanchard) infesting sugarcane crop was studied under laboratory conditions. In this bioassay Metarhizium anisopliae fungus caused higher rate of grub mortality at an overall concentration range of 4×105 to 2×106 conidia ml-1 as compared to Beauveria brongniartii and Beauveria bassiana The treatments with Metarhizium anisopliae fungus was found to be most effective and recorded 34.48-58.62 per cent grub mortality on 15 DAT at different conidial concentrations. The fungi Beauveria brongniartii recorded 31.03-55.18 per cent grub mortality, while as in case of fungi Beauveria bassiana 24.14-51.72 per cent grub mortality was observed on 15 DAT at different conidial concentrations. The estimated LC50 values for M. anisopliae, B. brongniartii and B. bassiana towards third instar grubs of Leucopholis lepidophora Blanch. Were 9.05×105,10.44×105 and 11.78×105 conidia ml-1, respectively.

Keywords

Metarhizium anisopliae, Beauveria brongniartii, Beauveria bassiana, Leucopholis lepidophora, Sugarcane
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  • Adsule, V.N. and Patil, S.M. (1990). Leucopholis lepidophora Bl. a new white grub pest of groundnut in western Maharashtra Groundnut News, pp. 2 : 7.

  • Agarwal, G.P. and Rajak, R.C. (1985). Entomopathogenic fungi in biological control of insect pests. Trends Pl. Res., pp. 34-42.

  • Ansari, M.A., Vestergaord, S. and Trirry, L. (2004). Selection of highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus. J. Invertebr. Path, 85 (2) : 89-96.

  • Bhagat, R.M., Gupta, R.B.L. and Yadav, C.P.S. (2003). Field efficacy of two entomopathogenic fungal formulations against white grub in Himachal Pradesh. Indian J. Entomol., 65 (1) : 76-81.

  • David, H., Nandgopal, V. and Anantnarayana, K. (1986). Recent studies on the control of white grubs, Holotrichia serrata Fabr. infesting sugarcane. J. Soil Biol. Ecol., 6 (2) : 117-127.

  • Dhoj, G.C.Y. and Keller, S. (2005). Metarhizium anisopliae for white grub control in Nepal Bulletin–OILB/SROP, 28 (2) : 57-61.

  • Finney, D.J. (1964). Pribit analysis. A statistical treatment of the sigmoid response curve. 2nd ed., Cambridge Univ. Press, London, 318 pp.

  • Gupta, R.B.L., Sharma, S. and Yadav, C.P.S. (2003). Effect of moisture regimes on efficacy of M. anisopliae against white grub (Holotrichia consanguinea). Indian J. Entomol., 65 (1) : 38-42.

  • Kulye, M.S. and Pokharkar, D.S. (2009). Evaluation of two species of entomopathogenic fungi against white grub, H. consanguinea (Blanch) infesting potato in Maharashtra. Indian J. Biol. Control., 23 (1) : 1-4.

  • Milner, R.J., Rogers, D.J., MCRac, C.M.N., Huppatz, R.J. and Brier, H. (1993). Preliminary evaluation of the use of Metarhizium anisopliae as a microbial for control of peanut scarabs. In: Pest control in sustainable agriculture. Melbourne, Australia, CSIRO, pp. 235-255.

  • Mishra, P.N. and Singh, M.P. (1999). Determination of predominant species of white grubs in Garhwal region of Uttar Pradesh Hills (India). J. Entomol. Res., 23:12-19.

  • Mohalkar, P.R., Patil, A.S., Shewale, B.S. and Hapse, D.G. (1997). White grub (Holotrichia serrata F.) The sixth joint convection of S.T.A.I., S.I.S.T.A. and D.S.T.A. pp. 67-77.

  • Najera–Rincon, M.B., Gracia-Martinez, M., Croker, R.L., Hernandez-velazquez, V. and Rodriguez-del-Bosque, L.A. (2005). Virulence of Beauveria bassiana and Metarhizium anisopliae from West Mexico against white grub third instar larvae Phyllophaga crinite (Coleoptera : Melolonthinae) under laboratory conditions. Fitosanidad., 9 (1) : 33-36.

  • Patil, S.M., Chauggle, C.B., Mohalkar, P.K., Ajri, D.S. and Patil, B.R. (1986). A new species of white grub, L. lepidoptera Blanchard infesting sugarcane in Kolhapur district. In : Abstract national seminar on pests and diseases management and national disorders in sugarcane, DSI, Pune, M.S. (INDIA).

  • Potter, D.A., Patterson, C.G. and Redmond, C.T. (1992). Influence of turf grass species and tall fescue endophyte on feeding ecology of Japanese beetle and southern masked chafer grubs (Coleoptera: Scarabaeidae). J. Economic. Entomol., 85 : 900-909.

  • Robertson, L.N., Kettle, C.G. and Bakker, P. (1997). Field evaluation of Metarhizium anisopliae for control of greyback canegrub (Dermolepida albohirtum) in north Queensland sugarcane Broc. Aust. Socc. Sugarcane Technol., 19 : 111-117.

  • Samson, P.R., Milner, R.J., Sander, E.D. and Bullard, G.K. (2005). Effect of fungicides and insecticides applied during planting of sugarcane on viability of Metarhizium anisopliae and its efficacy against white grub. Biocontrol, 50 (1) : 151-163.

  • Sharma, Shashi and Gupta R.B.L. (1998). Compatibility of Beauveria brongniartii with pesticides and organic manures. Pesticide Res. J., 10 (2) : 251-253.


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  • Bioefficacy of Different Species of Entomopathogenic Fungi Against White Grub, Leucopholis lepidophora (Blanchard) Infesting Sugarcane in Maharashtra

Abstract Views: 611  |  PDF Views: 0

Authors

Pradnya B. Mane
Division of Entomology, Mahatma Phule Krishi Vidyapeeth, College of Agriculture, Kolhapur (M.S.), India
Pandurang B. Mohite
Division of Entomology, Mahatma Phule Krishi Vidyapeeth, College of Agriculture, Kolhapur (M.S.), India

Abstract


The bioefficacy of three species of entomopathogenic fungi viz., Metarhizium anisopliae (Metsch.) Sorokin, Beauveria brongniartii (Sacc.) and Beauveria bassiana (Balsana) Vuillrmin, against white grub, Leucopholis lepidophora (Blanchard) infesting sugarcane crop was studied under laboratory conditions. In this bioassay Metarhizium anisopliae fungus caused higher rate of grub mortality at an overall concentration range of 4×105 to 2×106 conidia ml-1 as compared to Beauveria brongniartii and Beauveria bassiana The treatments with Metarhizium anisopliae fungus was found to be most effective and recorded 34.48-58.62 per cent grub mortality on 15 DAT at different conidial concentrations. The fungi Beauveria brongniartii recorded 31.03-55.18 per cent grub mortality, while as in case of fungi Beauveria bassiana 24.14-51.72 per cent grub mortality was observed on 15 DAT at different conidial concentrations. The estimated LC50 values for M. anisopliae, B. brongniartii and B. bassiana towards third instar grubs of Leucopholis lepidophora Blanch. Were 9.05×105,10.44×105 and 11.78×105 conidia ml-1, respectively.

Keywords


Metarhizium anisopliae, Beauveria brongniartii, Beauveria bassiana, Leucopholis lepidophora, Sugarcane

References