Asian Journal of Bio Science

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PCR Based Detection of Phytoplasma Association in Pot Marigold (Calendula officinalis L.) and Guldawari (Dendranthema grandiflora L.)


Affiliations
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
2 College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
     

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Flowering plants Calendula officinalis with phyllody and virescence and Dendranthema grandiflora with little leaf and formation of bladder like silique symptoms observed in Uttar Pradesh, India. The presence of phytoplasmas in diseased plants was detected by direct and nested polymerase chain reaction assays using phytoplasma-specific primer pairs P1/P7 and R16F2n/R2. In both flowering plants presence of phytoplasma was confirmed by amplification of 1200 bp product of phytoplasma 16S rRNA region with nested primer R16F2n/R2. This is the first report of phytoplasma associated with Calendula officinalis from India.

Keywords

Phytoplasma, Virescence, Phyllody, Nested PCR
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  • PCR Based Detection of Phytoplasma Association in Pot Marigold (Calendula officinalis L.) and Guldawari (Dendranthema grandiflora L.)

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Authors

Anchal Rani
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pragati Misra
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Jitendra Singh
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
Pankaj Kumar
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
Rosy Rani
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pradeep Shukla
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India

Abstract


Flowering plants Calendula officinalis with phyllody and virescence and Dendranthema grandiflora with little leaf and formation of bladder like silique symptoms observed in Uttar Pradesh, India. The presence of phytoplasmas in diseased plants was detected by direct and nested polymerase chain reaction assays using phytoplasma-specific primer pairs P1/P7 and R16F2n/R2. In both flowering plants presence of phytoplasma was confirmed by amplification of 1200 bp product of phytoplasma 16S rRNA region with nested primer R16F2n/R2. This is the first report of phytoplasma associated with Calendula officinalis from India.

Keywords


Phytoplasma, Virescence, Phyllody, Nested PCR

References