Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

PCR Based Detection of Phytoplasma Association in Pot Marigold (Calendula officinalis L.) and Guldawari (Dendranthema grandiflora L.)


Affiliations
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
2 College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
     

   Subscribe/Renew Journal


Flowering plants Calendula officinalis with phyllody and virescence and Dendranthema grandiflora with little leaf and formation of bladder like silique symptoms observed in Uttar Pradesh, India. The presence of phytoplasmas in diseased plants was detected by direct and nested polymerase chain reaction assays using phytoplasma-specific primer pairs P1/P7 and R16F2n/R2. In both flowering plants presence of phytoplasma was confirmed by amplification of 1200 bp product of phytoplasma 16S rRNA region with nested primer R16F2n/R2. This is the first report of phytoplasma associated with Calendula officinalis from India.

Keywords

Phytoplasma, Virescence, Phyllody, Nested PCR
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ajaykumar, P.V., Samad, A., Shasany, A.K., Gupta, M.K., Alam, M. and Rastogi, S. (2007). First record of a 'Condidatus phytoplasma' associated with little leaf disease of Portulaca grandiflora. Australasian Plant Disease Notes, 2 (1): 67-69

  • Chaturvedi, Y., Singh, M., Rao, G.P., Snehi, S.K., Raj, S.K. (2009a). First report of association of 'Candidatus phytoplasma asteris' (16SrI group) with little leaf disease of rose (Rosa alba) in India. Pl. Pathol., 58(4): 788.

  • Chaturvedi, Y., Tewari, A.K., Upadhyaya, P.P., Prabhuji, S.K., Rao, G.P. (2009b). Association of 'Candidatus phytoplasma asteris' with little leaf and phyllody disease of Catharanthus roseus in Eastern Uttar Pradesh, India. Medicinal Pl., 1(2): 103-108.

  • Chaturvedi, Y., Rao, G.P., Tewari, A.K., Duduk, B. and Bertaccini, A. (2010a). Phytoplasma in ornamentals: detection, diversity and management. Acta Phytopathologica et Entomologica Hungarica, 45(1): 31-69.

  • Deng, S. and Hiruki, D. (1991). Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. J. Microbiological Methods, 14 (1) : 53-61.

  • Efstratiou, E., Hussain, A.I., Nigam, P.S., Moore, J.E., Ayub, M.A. and Rao, J.R. (2012). Antimicrobial activity of Calendula officinalis petal extracts against fungi, as well as Gram-negative and Gram-positive clinical pathogens. Complement Ther. Clin. Pract., 18(3): 173-176.

  • Esmailzadeh-Hosseini, S.A, Salehi, M., Firooz, R. and Shamszadeh, M. (2008). Occurrence of marigold phyllody in Yazd province. 18th Iranian Plant Protection Congress. Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran, 418pp.

  • Esmailzadeh-Hosseini, S.A, Salehi, M., Khanchezar, A., Shamszadeh, M. (2011). The first report of a phytoplasma associated with pot marigold phyllody in Iran. Bulletin of Insectol., 64 (Supplement): 109-110.

  • Garcia-Chapa, M., Battle, A., Djaouida, R., Ruiz, M. and Firrao, G. (2004). PCR-mediated whole genome amplification of phytoplasmas. J. Microbiol. Methods., 56(2): 231-242.

  • Gundersen, D.E. and Lee, I.M. (1996). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea, 35(3): 144-151.

  • Jimenez-Medina, E., Garcia-Lora, A., Paco, L. et al. (2006). A new extract of the plant Calendula officinalis produces a dual in vitro effect: cytotoxic anti-tumor activity and lymphocyte activation. BMC Cancer, 6: 6.

  • Kumar, Ajay P.V., Samad, A., Shasany, A.K., Gupta, M.K., Alam, M. and Rastogi, S. (2007). First record of a 'Candidatus phytoplasma' associated with little leaf disease of Portulaca grandiflora. Australasian Pl. Disease Notes, 2(1): 67-69.

  • Kumar, S., Singh, V. and Lakhanpaul, S. (2012). First report of Mirabilis and Chrysanthemum little leaf associated with 'Ca. Phytoplasma aurantifolia' in India. Australasian Pl. Disease Notes, 7(1): 71-73.

  • Marcone, C., Ragozzino, A. and Seemuller, E. (1997). Detection and identification of phytoplasmas infecting vegetable, ornamental, and forage crops in southern Italy. J. Pl. Pathol., 79: 211-217.

  • McCoy, R.E., Caudwell, A., Chang, C.J., Chen, T.A., Chiykows, ki L.N., Cousin, M.T., Dale, J.L., de Leeuw, G.T.N., Golino, D.A., Hackett, K.J., Kirkpatrick, B.C., Marwitz, R., Petzold, H., Sinha, R.C., Sugiura, M., Whitcomb, R.F., Yang, I.L., Zhu, B.M. and Seemüller, E. (1989). Plant diseases associated with mycoplasma-like organisms. In: The Mycoplasmas, Whitcomb R.F., Tully J.G. (eds.), Academic Press, San Diego, 5 : 545-640.

  • Naito, T., Tanaka, M., Toyosato, S.T.T., Oshiro, A., Hokama, K.T.K., Usugi, T. and Kawano, S. (2007). Occurrence of chrysanthemum virescence caused by "Candidatus Phytoplasma aurantifolia" in Okinawa. J. General Pl. Pathol., 73(2): 139-141.

  • Raj, S.K., Khan, M.S. and Snehi, S.K. (2007a). Association of 'Candidatus Phytoplasma asteris' with little leaf disease of desert rose. Pl. Pathol., 56(6): 1040.

  • Raj, S.K., Khan, M.S. and Kumar, S. (2007b). Molecular identification of 'Candidatus Phytoplasma asteris' associated with little leaf disease of Chrysanthemum morifolium. Pl. Pathol., 2(1): 21-22.

  • Raj, S.K., Snehi, S.K., Kumar, S., Banerji, B.K., Dwivedi, A.K., Roy, R.K., Goel, A.K., (2009). First report of 'Candidatus phytoplasma asteris' (16SrI group) associated with colour-breaking and malformation of floral spikes of gladiolus in India. Pl. Pathol., 58(6): 1170.

  • Samad, A., Kumar, Ajay P.V., Shasany, A.K., Gupta, M.K., Alam, M., Rastogi, S. and Samad, A. (2008). Occurrence of a clover proliferation (16SrVI) group phytoplasma associated with little leaf disease of Portulaca grandiflora in India. Pl. Diseases, 92(5): 832.

  • Singh, M., Chaturvedi, Y., Tewari, A.K., Rao, G.P., Snehi, S.K., Raj, S.K. and Khan, M.S. (2011). Diversity among phytoplasmas infecting ornamental plants grown in India. Bulletin of Insectology, 64 (Supplement): S69-S70.

  • Smart, C.D., Schneider, B., Blomquist, C.L., Guerra, L.J., Harrison, N.A., Ahrens, U., Lorenz, K.H., Seemuller, E. and Kirkpatrick, B.C. (1996). Phytoplasma specific PCR primers based on sequences of the 16S-23S rRNA spacer region. Appl Environ. Microbiol., 62(8): 2988-2993.

  • Wang, K. and Hiruki, C. (2001). Molecular characterization and classification of phytoplasmas associated with canola yellows and a new phytoplasma strain associated with dandelions. Pl. Disease, 85(1): 76-79.


Abstract Views: 484

PDF Views: 0




  • PCR Based Detection of Phytoplasma Association in Pot Marigold (Calendula officinalis L.) and Guldawari (Dendranthema grandiflora L.)

Abstract Views: 484  |  PDF Views: 0

Authors

Anchal Rani
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pragati Misra
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Jitendra Singh
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
Pankaj Kumar
College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), India
Rosy Rani
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Pradeep Shukla
Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India

Abstract


Flowering plants Calendula officinalis with phyllody and virescence and Dendranthema grandiflora with little leaf and formation of bladder like silique symptoms observed in Uttar Pradesh, India. The presence of phytoplasmas in diseased plants was detected by direct and nested polymerase chain reaction assays using phytoplasma-specific primer pairs P1/P7 and R16F2n/R2. In both flowering plants presence of phytoplasma was confirmed by amplification of 1200 bp product of phytoplasma 16S rRNA region with nested primer R16F2n/R2. This is the first report of phytoplasma associated with Calendula officinalis from India.

Keywords


Phytoplasma, Virescence, Phyllody, Nested PCR

References