Asian Journal of Bio Science

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An Efficient Protocol for In Vitro Regeneration in Java Citronella (Cymbopogon winterianus) through Callus


Affiliations
1 Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
     

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The reproducible indirect in vitro regeneration system through callus was developed in Java citronella (Cymbopogon winterianus) for genotype Bio-13. Tender leaves from the lower portion of the citronella culms were used as explants. The MS medium supplemented with different concentrations of 2, 4-D (1.0, 3.0, 5.0 and 7.0 mg/l) alone or in combination of BAP (0.2, 0.4 and 0.6 mg/l) were used for callus induction. For regeneration of plantlets MS media with different concentrations of BAP (0.1, 0.2, 0.3 and 0.5 mg/l) alone or in combination with NAA (0.1, 0.2 and 0.3 mg/l) were employed. The induction of organogenic callus was highest in medium containing 5 mg/l 2, 4-D with 99.66 per cent explants showing callus formation. At higher concentration of BAP (0.5 mg/l) shoots were initiated rapidly from the callus within 13 days. The ischolar_main formation response was best in MS medium containing 3.0 mg/l NAA (83.33 % shoots formed ischolar_mains within 25 to 30 days). The regenerated plantlets transferred to autoclaved garden soil, soilrite and sand in 1:1:1 proportion and irrigated with half strength MS solution showed 85 per cent survival rate after three weeks.

Keywords

Callus, In-Vitro Regeneration, Aromatic Grass, Citronella, Somaclonal Variation.
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  • Be, L.V., Tan, V.T., Uyen, N.T.T. and Dung, L.V. (2008). Low-cost micropropagation of vetiver (Vetiveria zizanioides L.). Au. J. T., 12 (1): 18-24.

  • Cai, T. and Butler, L. (1990). Plant regeneration from embryogenic callus initiated from immature inflorescences of several high-tannin sorghums. Plant Cell Tiss. Org. Cult., 20: 101-110.

  • Denchev, P.D. and Conger, B.V. (1995). In vitro culture of switchgrass Influence of 2, 4-D and picloram in combination with benzyladenine on callus initiation and regeneration. Plant Cell Tiss. Org. Cult., 40: 43-48.

  • Hu, X.R., Yang, A.F., Zhang, K.W., Wang, J. and Zhang, J.R. (2006). Optimization of in vitro multiple shoot clump induction and plantlet regeneration of Kentucky bluegrass (Poapratensis). Plant Cell Tiss. Org. Cult., 84: 89–98.

  • Jain, M., Chengalrayan, K., Gallo-Meagherand, Maria and Mislevy, P. (2005). Embryogenic callus induction and regeneration in a pentaploid hybrid bermuda grass cv. Tifton 85. Crop Sci., 45: 1069-1072.

  • Lu, Y.C. and Vasil, I.K. (1981). Somatic embryogenesis and plant regeneration from leaf tissues of Panicum maximum Jacq. Theor. Appl. Genet., 59: 275-280.

  • Metzinger, B.D., Taliaferro, C.M., Johnson, B.B. and Mitchell, E.D. (1987). In vitro regeneration of apomictic bluestem grasses. Plant Cell Tiss. Org. Cult., 10: 31-38.

  • Murasighe, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15 : 473-497.

  • Nayak, S. (1996). Plant regeneration from callus culture of Cymbopogon (Jamrosa). J. Herbs, Spices & Medicinal Plants, 4 (2): 39-46.

  • Nayak, S., Debata, B.K. and Sahoo, S. (1996). Rapid propagation of lemon grass [Cymbopogon flexuosus (Nees) Wats.J through somatic embryogenesis in vitro. Plant Cell Rep., 15: 367-370.

  • Nirwan, R.S. and Kothari, S.L. (2003). High copper levels improve callus induction and plant regeneration in Sorghum bicolor (L.) In vitro cell dev. Biol-Plant, 39 (2): 161-164.

  • Panse V.G. and Sukhatme, P.V. (1956). Statistical analysis for agricultural workers. ICAR, NEW DELHI (INDIA).

  • Ramesh, M.V., Murugiah and Gupta, A.K. (2009). Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.). Indian J. Exp. Biol., 47: 68-74.

  • Saharan, V., Yadav, R.C., Yadav, Neelam R. and Chapagain, B.P. (2004). High frequency plant regeneration from desiccated calli of indicarice (Oryza sativa L.). Afr. J. Biotechnol., 3 (5): 256-259.

  • Patnaik J.S. and Debata, B.K. (1997). Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martinii). Plant Cell Rep., 16: 430-434.

  • Salehi, H., Salehi, M. and Ashiri, F. (2008). Some conditions for the best callus induction in common bermuda grass [Cynodondactylon (L.), Pers (californiya origin)]. Am-Euras. I. Agric. & Environ. Sci., 3 (3): 409-413.

  • Salisbury, F.B. and Ross, C.W. (2009). Plant physiology. Third Indian Reprint, Taj press. 367-385 pp.

  • Shiva, M.P, Lehri, A. and Shiva, Alka (2002). Aromatic and medicinal plant yielding essential oil for pharamaceutical, perfumery, cosmetic industries and trade. Internat. book distributors, 110-117 pp.

  • Sikder, M.B.H., Sen, P.K., Mamum, M.A., Ali, M.R. and Rahman, S.M. (2006). In vitro regeneration of aromatic rice (Oryzasativa L.) Int. J. Agri. Biol., 8 (6): 759-762.

  • Sreenath, H.L. and Jagadishchandra, K.S. (1989). Somatic embryogenesis and plant regeneration from inflorescence culture of Java citronella (Cymbopogon winterianus). Annal. Bot., 64: 211-215.

  • Tariq, M., Ali, G., Hadi, F., Ahmad, S., Ali, N. and Shah, A.A. (2008). Callus induction and in vitro plant regeneration of rice (Oryza sativa L.) under various conditions. Pak. J. Biol. Sci., 11: 255-259.


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  • An Efficient Protocol for In Vitro Regeneration in Java Citronella (Cymbopogon winterianus) through Callus

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Authors

Santosh J. Gahukar
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
Snehal M. Bansod
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
Amrapali A. Akhare
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India

Abstract


The reproducible indirect in vitro regeneration system through callus was developed in Java citronella (Cymbopogon winterianus) for genotype Bio-13. Tender leaves from the lower portion of the citronella culms were used as explants. The MS medium supplemented with different concentrations of 2, 4-D (1.0, 3.0, 5.0 and 7.0 mg/l) alone or in combination of BAP (0.2, 0.4 and 0.6 mg/l) were used for callus induction. For regeneration of plantlets MS media with different concentrations of BAP (0.1, 0.2, 0.3 and 0.5 mg/l) alone or in combination with NAA (0.1, 0.2 and 0.3 mg/l) were employed. The induction of organogenic callus was highest in medium containing 5 mg/l 2, 4-D with 99.66 per cent explants showing callus formation. At higher concentration of BAP (0.5 mg/l) shoots were initiated rapidly from the callus within 13 days. The ischolar_main formation response was best in MS medium containing 3.0 mg/l NAA (83.33 % shoots formed ischolar_mains within 25 to 30 days). The regenerated plantlets transferred to autoclaved garden soil, soilrite and sand in 1:1:1 proportion and irrigated with half strength MS solution showed 85 per cent survival rate after three weeks.

Keywords


Callus, In-Vitro Regeneration, Aromatic Grass, Citronella, Somaclonal Variation.

References