Asian Journal of Bio Science

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An Efficient Protocol for In Vitro Regeneration in Java Citronella (Cymbopogon winterianus) through Callus


Affiliations
1 Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
     

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The reproducible indirect in vitro regeneration system through callus was developed in Java citronella (Cymbopogon winterianus) for genotype Bio-13. Tender leaves from the lower portion of the citronella culms were used as explants. The MS medium supplemented with different concentrations of 2, 4-D (1.0, 3.0, 5.0 and 7.0 mg/l) alone or in combination of BAP (0.2, 0.4 and 0.6 mg/l) were used for callus induction. For regeneration of plantlets MS media with different concentrations of BAP (0.1, 0.2, 0.3 and 0.5 mg/l) alone or in combination with NAA (0.1, 0.2 and 0.3 mg/l) were employed. The induction of organogenic callus was highest in medium containing 5 mg/l 2, 4-D with 99.66 per cent explants showing callus formation. At higher concentration of BAP (0.5 mg/l) shoots were initiated rapidly from the callus within 13 days. The ischolar_main formation response was best in MS medium containing 3.0 mg/l NAA (83.33 % shoots formed ischolar_mains within 25 to 30 days). The regenerated plantlets transferred to autoclaved garden soil, soilrite and sand in 1:1:1 proportion and irrigated with half strength MS solution showed 85 per cent survival rate after three weeks.

Keywords

Callus, In-Vitro Regeneration, Aromatic Grass, Citronella, Somaclonal Variation.
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  • An Efficient Protocol for In Vitro Regeneration in Java Citronella (Cymbopogon winterianus) through Callus

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Authors

Santosh J. Gahukar
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
Snehal M. Bansod
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
Amrapali A. Akhare
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India

Abstract


The reproducible indirect in vitro regeneration system through callus was developed in Java citronella (Cymbopogon winterianus) for genotype Bio-13. Tender leaves from the lower portion of the citronella culms were used as explants. The MS medium supplemented with different concentrations of 2, 4-D (1.0, 3.0, 5.0 and 7.0 mg/l) alone or in combination of BAP (0.2, 0.4 and 0.6 mg/l) were used for callus induction. For regeneration of plantlets MS media with different concentrations of BAP (0.1, 0.2, 0.3 and 0.5 mg/l) alone or in combination with NAA (0.1, 0.2 and 0.3 mg/l) were employed. The induction of organogenic callus was highest in medium containing 5 mg/l 2, 4-D with 99.66 per cent explants showing callus formation. At higher concentration of BAP (0.5 mg/l) shoots were initiated rapidly from the callus within 13 days. The ischolar_main formation response was best in MS medium containing 3.0 mg/l NAA (83.33 % shoots formed ischolar_mains within 25 to 30 days). The regenerated plantlets transferred to autoclaved garden soil, soilrite and sand in 1:1:1 proportion and irrigated with half strength MS solution showed 85 per cent survival rate after three weeks.

Keywords


Callus, In-Vitro Regeneration, Aromatic Grass, Citronella, Somaclonal Variation.

References