Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Laboratory Evaluation of Entomopathogenic Fungus Alone or in Combination with Edible Oils on Progeny Adult Build-up of Lesser Grain Borer on Stored Paddy


Affiliations
1 Department of Entomology, Agricultural College Campus, Bapatla (A.P.), India
2 Post Harvest Technology Centre, Agricultural College Campus, Bapatla (A.P.), India
3 Microbiology Laboratory, Agricultural College Campus, Bapatla (A.P.), India
     

   Subscribe/Renew Journal


The entomopathogenic fungi, Beauveria bassiana (2 × 106 conidia/g), Metarhizium anisopliae (1 × 109 conidia/g) and Lecanicillium lecanii (2 × 107 conidia/g) @ 5 g/l as bag treatment and 5g/kg of paddy as grain treatment alone, their interactions and the compatibility of entomopathogenic fungi @ 5g/kg with two vegetable oils (2 ml/kg) viz., sunflower oil and groundnut oil were tested against the progeny build-up of lesser grain borer, R. dominica. In the grain treatment, least progeny of 122.33 was observed with B. bassiana followed by M. anisopliae (130.67) which were at par at 180 DAT. Among the bag treatment, M. anisopliae recorded the lower progeny of 266.33 followed by B. bassiana (291.00) and L. lecanii (298.67) which were at par with each other but were significantly different from control (366.33). In the study of interaction effects, Beauveria + Metarhizium + Lecanicillium had recorded least progeny of 119.67 followed by Beauveria + Metarhizium (122.00) and were superior over all other treatments. In the study of compatibility of entomopathogenic fungi with edible oils, progeny build up recorded at 180 DAT was found to be less with Beauveria + Groundnut oil (118.33) followed by Metarhizium + Groundnut oil (121.33) compared to oils alone, sunflower (307.67) and groundnut (252.33) but were significantly different from control (517.00).

Keywords

Beauveria, Lecanicillium, Metarhizium, Rhyzopertha dominica, Sunflower Oil
Subscription Login to verify subscription
User
Notifications
Font Size


  • Arthur, F.H., Bautista, R.C and Siebenmorgen, T.J. (2007). Influence of growing location and cultivar on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) infestation of rough rice. Insect Sci., 14(3): 231-239.

  • Batta, Y.A. (2005). Control of lesser grain borer [Rhyzopertha dominica (F.), Coleoptera: Bostrichidae] by treatments with residual formulations of Metarhizium anisoplea (Metschnikoff) Sorokin. J. Stored Products Res., 41(2): 221-229.

  • El-Sebai, O.A. (2011). Laboratory assessment of entomopathogenic fungi Beauveria bassiana (Balsamo) to control the grain borer Rhyzopertha dominica (F.) on wheat kernels. J. Plant Protec. & Pathol., 2 (2): 225-237.

  • Hafez, S.F. (2011). Efficacy of the entomopathogenic fungus Beauveria bassiana (Balsamo) against Tribolium confusum (Duval) on stored wheat flour. J. Plant Prot. & Pathol., 2(2): 203-211.

  • Kavallieratos, N.G., Athanassiou, C.G., Michalaki, M.P., Batt, Y.A., Rigatos, A., Pashalidou, F.G., Balotis, N., Tomanovic, Z. and Vayias, B.J. (2006). Effect of the combined use of Metarhizium anisoplea (Metschinkoff) sorokin and diatomaceous earth for the control of 3 stored product beetles. Crop Prot., 25(10): 1087-1094.

  • Khalequzzaman, M., Mahdi, S.H.A. and Osman Goni, S.H.M. (2007). Efficacy of edible oils in the control of pulse beetle, Callosobruchus chinensis L. in stored pigeonpea. Univ. J. Zoo., Rajshahi University, 26: 89-92.

  • Padin, S., Dal Bello, G. and Fabrizio, M. (2002). Grain loss caused by Tribolium castaneum, Sitophilus oryzae and Acanthoscelides obtectus in stored durum wheat and beans treated with Beauveria bassiana. J. Stored Products Res., 38(1): 69-74.

  • Pedrini, N., Villaverde, M.L., Fuse, C.B, Dal Bello, G. and Juarez, M.P. (2010). Beauveria bassiana infection alters colony of the beetles Tribolium castaneum and Ulomoides dermestoides (Coleoptera: Tenebrionidae). J. Econ. Entomol., 103(4): 1094-1099.

  • Rice, W.C and Cogburn, R.R. (1999). Activity of the entomopathogenic fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) against three coleopteran pests of stored grain. J. Econ. Entomol., 92(3): 691-694.

  • Sabbour, M.M. and Shadia, E. (2007). Efficiency of some bioinsecticides against broad bean beetle, Bruchus rufimanus (Coleoptera: Bruchidae). Res. J. Agric. & Biol. Sci., 3(2): 67-72.

  • Sheeba, G., Sundaram, S., Nagappan, R., Janarthanan, S. and Savarimuthu, I. (2001). Efficacy of Beauveria bassiana for control of the rice weevil Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Appl. Entomol. & Zool., 36(1): 117-120.

  • Swaminathan, M. (1977). Effect of insect infestation on weight loss, hygienic condition, acceptability and nutritive value of food grains. Indian J. Nutr. & Dietetics. 14: 205-216.

  • Tahira, R., Wakil, W., Muhammad, A, and Shahbaz, T.S. (2011). Effect of Beauveria bassiana mixed with diatomaceous earth on mortality, mycosis and sporulation of Rhyzopertha dominica on stored wheat. Phytoparasitica., 39(4): 325-331.

  • Throne, J.E. and Lord, C.J. (2004). Control of sawtoothed grain beetles (Coleoptera: Silvanidae) in stored oats by using an entomopathogenic fungus in conjunction with seed resistance. J. Econ. Entomol., 97(5): 1765-1771.

  • Wakefield, M.E. (2006). Factors affecting storage insect susceptibility to the entomopathogenic fungus Beauveria bassiana. 9th International Working Conference on Stored Product Protection. PS7-27-6250: 885-862.

  • Williams, H.J., Silverstein, R.M., Burkholder, W.E and Khorramshahi, A. (1981). Dominicalure 1 and 2: components of aggregation pheromone from male lesser grain borer, Rhyzopertha dominica (Coleoptera: Bostrichidae). J. Chemical Ecol., 7: 759-780.

  • Zimmermann, G. (2007a). Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Sci. & Tech., 17(6): 553-596.

  • Zimmermann, G. (2007b). Review on safety of the entomopathogenic fungus Metarhizium anisopliae. Biocontrol Sci. & Tech., 17(9): 879-920.


Abstract Views: 435

PDF Views: 1




  • Laboratory Evaluation of Entomopathogenic Fungus Alone or in Combination with Edible Oils on Progeny Adult Build-up of Lesser Grain Borer on Stored Paddy

Abstract Views: 435  |  PDF Views: 1

Authors

P. Jyothi
Department of Entomology, Agricultural College Campus, Bapatla (A.P.), India
N. Sambasiva Rao
Post Harvest Technology Centre, Agricultural College Campus, Bapatla (A.P.), India
R. Lakshmipathy
Microbiology Laboratory, Agricultural College Campus, Bapatla (A.P.), India

Abstract


The entomopathogenic fungi, Beauveria bassiana (2 × 106 conidia/g), Metarhizium anisopliae (1 × 109 conidia/g) and Lecanicillium lecanii (2 × 107 conidia/g) @ 5 g/l as bag treatment and 5g/kg of paddy as grain treatment alone, their interactions and the compatibility of entomopathogenic fungi @ 5g/kg with two vegetable oils (2 ml/kg) viz., sunflower oil and groundnut oil were tested against the progeny build-up of lesser grain borer, R. dominica. In the grain treatment, least progeny of 122.33 was observed with B. bassiana followed by M. anisopliae (130.67) which were at par at 180 DAT. Among the bag treatment, M. anisopliae recorded the lower progeny of 266.33 followed by B. bassiana (291.00) and L. lecanii (298.67) which were at par with each other but were significantly different from control (366.33). In the study of interaction effects, Beauveria + Metarhizium + Lecanicillium had recorded least progeny of 119.67 followed by Beauveria + Metarhizium (122.00) and were superior over all other treatments. In the study of compatibility of entomopathogenic fungi with edible oils, progeny build up recorded at 180 DAT was found to be less with Beauveria + Groundnut oil (118.33) followed by Metarhizium + Groundnut oil (121.33) compared to oils alone, sunflower (307.67) and groundnut (252.33) but were significantly different from control (517.00).

Keywords


Beauveria, Lecanicillium, Metarhizium, Rhyzopertha dominica, Sunflower Oil

References