Selection of Influential Parameters for Bacteriocin Production by Lactococcus Lactis Subsp. Lactis R10 by Plackett-Burman Design

In the present study, Plackett-Burman design was applied to select influential parameters for bacteriocin production by Lactococcus lactis subsp. lactis R10, an isolate from fermented radish was investigated. Out of the eleven culture media constituents screened, six constituents namely temperature, dipotassium hydrogen phosphate, triammonium citrate, sodium acetate, yeast extract and pH were found to contribute positively to the overall bacteriocin production. Sucrose, peptone, magnesium sulphate, tween 80 and glycerol had a negative effect on bacteriocin production by L. lactis subsp. lactis R10. Thus, the statistical approach employed in this study allows for rapid identification of important culture media parameters affecting the bacteriocin production.