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Selection of Influential Parameters for Bacteriocin Production by Lactococcus Lactis Subsp. Lactis R10 by Plackett-Burman Design
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In the present study, Plackett-Burman design was applied to select influential parameters for bacteriocin production by Lactococcus lactis subsp. lactis R10, an isolate from fermented radish was investigated. Out of the eleven culture media constituents screened, six constituents namely temperature, dipotassium hydrogen phosphate, triammonium citrate, sodium acetate, yeast extract and pH were found to contribute positively to the overall bacteriocin production. Sucrose, peptone, magnesium sulphate, tween 80 and glycerol had a negative effect on bacteriocin production by L. lactis subsp. lactis R10. Thus, the statistical approach employed in this study allows for rapid identification of important culture media parameters affecting the bacteriocin production.
Keywords
Lactococcus lactis subsp. lactis R10, Bacteriocin, Plackett-Burman Design
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- Antony, J. (2008). Design of experiments for engineers and scientists. Butterworth Heinemann, U.K., pp. 44-53.
- Chauhan, K., Trivedi, U. and Patel, K.C. (2007). Statistical screening of medium components by Plackett-Burman design for lactic acid production by Lactobacillus sp. KCP01 using date juice. Biores. Technol., 98(1): 98-103.
- Cleveland, J., Montville, T.J., Nes, I.F. and Chikindas, M.L. (2001). Bacteriocins: safe, natural antimicrobials for food preservation. Internat. J. Food Microbiol., 71(1): 1-20.
- De Vuyst, L. and Vandamme, E.J. (1993). Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp. lactis batch fermentation using a complex medium. J. Appl. Microbiol. Biotechnol., 40(1): 17-22.
- De Vuyst, L., Callewaert, R. and Crabbe, K. (1996). Primary metabolite kinetics of bacteriocin biosynthesis by Lactobacillus amylovorus and evidence for stimulation of bacteriocin production under unfavourable growth conditions. Microbiol., 142(4): 817-827.
- Delgado, A., Brito, D., Peres, C., Noe-Arroyo, F. and Garrido-Fernandez, A. (2005). Bacteriocin production by Lactobacillus pentosus B96 can be expressed as a function of temperature and NaCl concentration. Food Microbiol., 22(6): 521-528.
- Guo, W.I., Zhang, Y.B., Lu, J.H., Jiang, L.Y., Teng, L.R., Wang, Y. and Liang. Y.C. (2010). Optimization of fermentation medium for nisin production from Lactococcus lactis subsp. lactis using response surface methodology (RSM) combined with artificial neutral network- genetic algorithm (ANN-GA). Afr. J. Biotechnol., 9(38): 6264-6272.
- Haaland, P.D. (1989). Experimental design in biotechnology. Marcel Dekker Inc., New York.
- Hugas, M., Garriga, M., Pascual, M., Aymerich, M.T. and Monfort, J.M. (2002). Enhancement of sakacin K activity against Listeria monocytogenes in fermented sausages with pepper or manganese as ingredients. Food Microbiol., 19(5): 519-528.
- Kanmani. P., Sathishkumar, R., Yuvaraj, N., Paari, K.A., Pattukumar, V. and Arul, V. (2011). The role of environmental factors and medium composition on bacteriocin production by an aquaculture probiotic Enterococcus faecium MC13 isolated from fish intestine. Korean. J. Chem. Engg., 28(3): 860-866.
- Kumar, M. and Srivastava, S. (2010). Enhanced production of enterocin from Enterococcus faecium LR/6 by statistical optimization of the growth medium. Trakia J. Sci., 8(4): 12-21.
- Leroy, F. and De Vuyst, L. (1999). Temperature and pH conditions that prevail during the fermentation of sausages are optimal for production of the antilisterial bacteriocin sakacin K. Appl. Environ. Microbiol., 65 (3) : 974-981.
- Mackay. V.C., Arendse, G. and Hastings, J.W. (1997). Purification of bacteriocins of lactic acid bacteria: problems and pointers. Internat. J. Food Microbiol., 34 (1) : 1-16.
- Meng, Q., Cai, Q., Shi, B., Fu, R., Li, J., Chen, X., Qi, K. and Zhang, M. (2012). Optimization of medium composition for production of lacticin LLC518 by Lactococcus lactis subsp. lactis LLC518 using response surface methodology. J. Food Agric. Environ., 10(1): 137-142.
- Nilsson, L., Nielson, M.K., Yin, Ng and Lone, Gram. (2002). Role of acetate in production of an autoinducible class IIa bacteriocin in Carnobacterium piscicola A9b. Appl. Environ. Microbiol., 68(5): 2251-2260.
- Oh, S., Rheem, S., Sim, J., Kim, S. and Baek, Y. (1995). Optimizing conditions for the growth of Lactobacillus casei YIT 9018 in tryptone-glucose medium by using response surface methodology. Appl. Environ. Microbiol., 61(11): 3809-3814.
- Plackett, R.L. and Burman, J.P. (1946). The design of optimum multifactorial experiments. Biometrika, 33(4): 305-25.
- Settanni, L., Valmorri, S., Suzzi, G. and Corsetti, A. (2008). The role of environmental factors and medium composition on bacteriocin like inhibitory substances (BLIS) production by Enterococcus mundtii strains. Food Microbiol., 25(5): 722-728.
- Sharma, D.C. and Satyanarayana, T. (2006). A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus dcsr1 in submerged fermentation by using statistical methods. Bioresour. Technol., 97(5): 727-733.
- Tagg, J.R. and Mc Given, A.R. (1971). Assay system for bacteriocins. Appl. Microbiol., 21(5): 943-949.
- Todorov, S.D. and Dicks, L.M.T. (2005). Lactobacillus plantarum isolated from molasses produces bacteriocins active against Gram Negative bacteria. Enzyme Microb. Technol., 36(2-3): 318-326.
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