Asian Journal of Bio Science

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Effect of Cold Pre-treatment on Another Culture in Different Brassica Genotypes


Affiliations
1 School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology (J) Chatha, Jammu (J.&K.), India
     

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Stress is an essential component to enhance callus induction in anther culture. Cold pretreatment has been used in cereal crops but very seldom attempted in Brassica anther culture. In order to assess the effect of cold pretreatment (4°C) of flower buds subjected to a liquid medium for 5, 10 and 12 days for callus induction in different genotypes of Brassica, three varieties (GSL1, DGS1 and RSPN25) of B. napus and three varieties of Brassica juncea (RSPR01, Varuna and Kranti) were evaluated. The appropriate duration of cold pre-treatment of flower buds was found to be 10 days which stimulated the maximum callus induction. Cold pretreatment was also able to promote development including the improvement of embryo quality and acceleration of embryogenesis.

Keywords

Haploids, Brassica napus, Brassica juncea, Embryogenesis, Organogenesis
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  • Effect of Cold Pre-treatment on Another Culture in Different Brassica Genotypes

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Authors

Preeti Kumari
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology (J) Chatha, Jammu (J.&K.), India
A.K. Singh
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology (J) Chatha, Jammu (J.&K.), India

Abstract


Stress is an essential component to enhance callus induction in anther culture. Cold pretreatment has been used in cereal crops but very seldom attempted in Brassica anther culture. In order to assess the effect of cold pretreatment (4°C) of flower buds subjected to a liquid medium for 5, 10 and 12 days for callus induction in different genotypes of Brassica, three varieties (GSL1, DGS1 and RSPN25) of B. napus and three varieties of Brassica juncea (RSPR01, Varuna and Kranti) were evaluated. The appropriate duration of cold pre-treatment of flower buds was found to be 10 days which stimulated the maximum callus induction. Cold pretreatment was also able to promote development including the improvement of embryo quality and acceleration of embryogenesis.

Keywords


Haploids, Brassica napus, Brassica juncea, Embryogenesis, Organogenesis

References