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Helping Hand of Biotechnology in Conservation of Medicinally Important Plant (Asparagus racemosus)
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Increase in human population, urbanization, cutting trees and use of plants for pharmaceutical purpose leads to destruction of medicinal plant. Not only destruction but some of the species become threaten for their existence. One of them is Asparagus racemosus whose ischolar_mains and its extracts widely used for the preparation of medicines by the pharmaceutical companies. That's why, conservation efforts have been required which can be achieved with the help of various plant biotechnological techniques such as suspension culture. Not only for the conservation, it provides an alternative way of producing large amount of active compound directly in to the laboratory. For the establishment of suspension culture techniques a prime requirement is to have a standardize protocol for callus culture. This paper represents optimization of protocol for callus culture and suspension culture from Asparagus racemosus. It has been proved that the plant hormones play vital role for callus induction. In this work MS Medium with different combination of auxin (NAA, 2, 4 D) and cytokinines (BAP, Zeatin, Kinetin) have been tried for callus induction. The best friable callus induction, highest callus induction rate and excellent callus growth were obtained in MS medium supplemented with 1.0mg/L ZEATIN + 0.1mg/L BAP. Same medium was then utilized for the establishment of suspension culture. In cell suspension culture of A. racemosus, the maximum growth (7.7 g/l) were observed in suspension culture initiated from leaf explant and the highest saponin production (0.580 mg/g) were obtained from the biomass of suspension culture initiated from ischolar_main explant at 18 days after culture.
Keywords
Callus Culture, Suspension Culture, Secondary Metabolites.
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- Behbahani, M., Shanehsazzadeh, M. and Hessami, M.J. (2011). Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production, (February), pp.69–76.
- Bopana, N. and Saxena, S. (2007). Asparagus racemosus – ethnopharmacological evaluation and conservation needs. J. ethnopharmacology, 110(1): 1–15.
- Bopana, N. and Saxena, S. (2008). In vitro opagation of a high value medicinal plant: Asparagus racemosus Willd. In Vitro Cellular & Developmental Bio. -Pl., 44(6): 525–532.
- Castellar, A., Gagliardi, R.F. and Mansur, E. (2011). In vitro propagation and establishment of callus and cell suspension cultures of Petiveria alliacea L., A Valuable Medicinal Plant, 5(7): 1113–1120.
- Chattopadhyay, S. et al. (2002). Bioprocess considerations for production of secondary metabolites by plant cell suspension cultures. Biotechnology & Bioprocess Engineering, 7(3): 138–149.
- Crops, V. et al. (2000). Vegetable crops production guide for the atlantic provinces.
- Firdaus, M.T., Izam, A. and Resources, N. (2010). Ultrasonic-assisted extraction of triterpenoid saponins from mangrove leaves., 1: 1–8.
- Grzegorczyk, I., Królicka, A. and Wysokinska, H. (2006). Establishment of Salvia officinalis L. hairy ischolar_main cultures for the production of rosmarinic acid. Zeitschrift für Naturforschung. C. J. Biosciences, 61(5-6): 351–356.
- Haralampidis, K., Trojanowska, M. and Osbourn, A.E. (2002). Biosynthesis of triterpenoid saponins in plants. Advances in biochemical engineering/biotechnology, 75: 31–49.
- Karuppusamy, S. (2009). A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J. Med. Pl. Res., 3(13): 1222–1239.
- Mehta, S.R. and Subramanian, R.B. (2005). Direct in vitro propagation of Asparagus adscendens Roxb., Plant Tissue Cult., 15(1): 25–32.
- Mulabagal, V. and Tsay, H. (2004). Plant Cell Cultures - An Alternative and Efficient Source for the Production of Biologically Important Secondary Metabolites, pp.29–48.
- Nalawade, S.M. and Tsay, H.S. (2004). In vitro propagation of some important chinese medicinal plants and their sustainable usage. In Vitro Cellular & Developmental Bio.Pl., 40(2): 143–154.
- Pant, K.K. and Joshi, S.D. (2009). In vitro Multiplication of Wild Nepalese Asparagus racemosus Through Shoots and Shoot Induced Callus Cultures. Bot. Res. Internat., 2(2): 88–93.
- Schmeda-Hirschmann, G., Jordan, M., Gerth, A., Wilken, D., Hormazabal, E. and Tapia, AA. (2004). Secondary metabolite content in Fabiana imbricata plants and in vitro cultures. Zeitschrift fuer Naturforschung. C, J. Biosciences, 59(1-2): 48–54.
- Sharan, M., Nene, C. and Sharon, M. (2011). Regeneration of Asparagus racemosus by shoot apex and nodal explants. Asian J. Plant Sci. & Res., 1(2): 49–56.
- Sinha, S.N. and Biswas, Mrinal (2011). Effect of Extracts from Asparagus racemosus willd ischolar_main against pathogenic bacteria. Internat. J. Appl. Biol. & Pharmaceutical Technol., 2(3): 312–314.
- Soomro, R. and Memon, R.A. (2007). Establishment of callus and suspension, 39(7): 2431–2441.
- Sujanya, S., Devi, B.P. and Sai, I. (2008). In vitro production of azadirachtin from cell suspension cultures of Azadirachta indica. J. Biosciences, 33(1): 113–120.
- Tripathi, L. and Tripathi, J.N. (2003). Role of biotechnology in medicinal plants. Trop. J. Pharm. Res., 2(2): 243–253.
- Vanisree, M. Chen-Yue Lee, Shu-Fung Lo, Satish Manohar Nalawade, Chien Yih Lin and Hsin-Sheng Tsay (2004). Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures. Bot. Bull. Acad.. Sin., 45: 1-22.
- Xu, Hui, Yong Kyoung Kim, Xuanji Jin, Sook Young Lee and Sang Un Park (2008). Rosmarinic acid biosynthesis in callus and cell cultures of Agastache rugosa Kuntze, J. Med. Pl. Res., 2(9): 237–241.
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