Asian Journal of Bio Science

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Genetic Diversity Analysis of Pumpkin Genotypes (Cucurbita moschat Duch ex. Poir) using Morphological and Rapd Markers


Affiliations
1 Department of Horticulture, University of Agricultural Sciences, Bengaluru (Karnataka), India
     

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The study was conducted to ascertain genetic diversity of 20 pumpkin (Cucurbita moschata Duch ex. Poir) genotypes collected from different parts of India, using morphological and random amplified polymorphic DNA (RAPD) markers. The morphological data recorded for growth and yield characters at different levels. CTAB method used for isolation of DNAs from 20 genotypes. A total of 21 markers produced form 3 primers, out of that one band was polymorphic and remaining once were monomorphic. A dendogram grouped the genotypes into 2 clusters A and B at 9 linkage distance. Cluster B was the major group consisted of 12 accessions, CM-14 genotype which is diverse form other genotypes grouped under cluster B. Cluster B again sub divided into 6 sub groups. The second cluster A consisted of 8 accessions separated at 3 linkage distance and divided into 2 groups. The genetic dissimilarity matrix based on Squared Euclidean Distance, showed a maximum dissimilarity (10%) between the genotypes Bangalore local-2 and Magadi local and the minimum dissimilarity (1%) between CO-2 and Solan Badam, CO-2 and Bangalore local-1, KIC-1 and IIHR-5. This analysis showed the potentiality of CM-14 variety due to its less vine length, less number of days to female flower appearance and early maturity as probable parent in hybridization programme.

Keywords

Cluster Analysis, CTAB, Dissimilarity Matrix, Genetic Diversity, RAPD.
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  • Bose, T.K. and Som, M.C. (1990). Vegetable crops in India, 1st reprint, Good Association Printers, Calcutta. pp.148-150.

  • Chowdhury, A.K., Srinivar, P., Tongpamnak, P. and Saksoong, P. (2001). Genetic diversity based on morphology and RAPD analysis in vegetable soybean. Korean J. Crop Sci., 46: 112-120.

  • Dey, S.S., Singh, A.K., Chandal, D. and Behera, T.K. (2006). Genetic diversity of bitter gourd (Momordica charantia L.) genotypes revealed by RAPD markers and agronomic traits. Sci. Hort., 109 (1): 21-28.

  • Doijode, S.D. and Sulladmath, U.V. (1986). Genetic variability and correlation studies in pumpkin (Cucurbita moschata Poir.). Mysore J. Agri. Sci., 29: 59-61.

  • Dahlberg, J.A. (2000). Classification and characterization of sorghum. In: Smith, C.W., Ferderiksen, R.A. (Eds), Sorgham: Origin, history, technology and production. John Wiley and Sons, Inc., New York, pp 99-130.

  • Ferriol, M., Pico, M.B. and Nuez, F. (2003). Genetic diversity of some accessions of Cucurbita maxima from Spain using RAPD and SBAP markers. Genetic Resour. & Crop-Evolution, 50(3): 227-238.

  • Gopalakrishnan, T.R., Gopalakrishnan, K.G. and Peter, K.V. (1980). Variability, heritability and correlation among some polygenic characters in pumpkin. Indian J. Agric. Sci., 50(12): 925-930.

  • Gwanama, C., Labuschagne, M.T. and Botha, A.M. (2000). Analysis of genetic variation in Cucurbita moschata by random amplified polymorphic DNA (RAPD) markers. Euphytica, 113 (1): 19-24.

  • Horejsi, T. and Stub, J.E. (1999). Genetic variation in cucumber (Cucumis sativus L.) asassed by random amplified polymorphic DNA. Genetic & Crop Evolution, 46 (4): 337-350.

  • Jack, E., Staub Ana, I.L., Opez-ses, E. and Nikolaos, Fanourakis. (2004). Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica, 136 (2) : 151–166.

  • Kumar, R.J. (1982). Variability and correlation studies in pumpkin (Cucurbita moschata Poir). M.Sc. (Hort.) Thesis, Haryana Agricultural University, Hissar, HARYANA (INDIA).

  • Lakshmi, L.M., Haribabu, K. and Reddy, G.L.K. (2002). Genetic variability studies in pumpkin (Cucurbita moschata Duch ex. poir). J. Res., ANGRAU, 30(1): 82-86.

  • Ortiz, R. (1997). Morphological variation in Musa germplasm. Genetic Res. & Crop Evolution, 44 (5): 393-402.

  • Rafalski, J.A. and Tingey, S.V. (1993). Genetic diagnosis in plant breeding: RAPDs microsatellite and machines. Trends Genet., 9 (8): 275-280.

  • Simon, L., Shyamalamma, S. and Narayanaswamy, P. (2007). Morphological and molecular analysis of genetic diversity in jack fruit. J. Hort. Sci. & Biotec., 82(5): 764-768.

  • Rathod, Vijaykumar (2007). Studies on genetic variability and molecular characterization of bitter gourd (Momordica charantia L.) genotypes. M.Sc. (Hort.) Thesis, University of Agricultural Sciences, Bengaluru, KARNATAKA (INDIA).

  • Williams, J.G.K., Kubelik, A.R., Livik, K.J., Rafalski, J.A. and Tingery, S.V. (1990). DNA polymorphisms amplified arbitrary primers are useful as genetic markers. Nucleic Acids, 18 (22): 6531-6535.

  • Ward, J.H. (1963). Hierarchic grouping to optimize an objective function. J. American Stat. Assoc., 58: 236-239.

  • Youn, S.J. and Chung, H.D. (1998). Genetic relationship among the local varieties of the Korean native squashes (Cucurbita moschata) using RAPD technique. J. Korean Soc. Hort. Sci., 39 (5): 517-521.


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  • Genetic Diversity Analysis of Pumpkin Genotypes (Cucurbita moschat Duch ex. Poir) using Morphological and Rapd Markers

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Authors

M. S. Muralidhara
Department of Horticulture, University of Agricultural Sciences, Bengaluru (Karnataka), India
N. C. Narasegowda
Department of Horticulture, University of Agricultural Sciences, Bengaluru (Karnataka), India

Abstract


The study was conducted to ascertain genetic diversity of 20 pumpkin (Cucurbita moschata Duch ex. Poir) genotypes collected from different parts of India, using morphological and random amplified polymorphic DNA (RAPD) markers. The morphological data recorded for growth and yield characters at different levels. CTAB method used for isolation of DNAs from 20 genotypes. A total of 21 markers produced form 3 primers, out of that one band was polymorphic and remaining once were monomorphic. A dendogram grouped the genotypes into 2 clusters A and B at 9 linkage distance. Cluster B was the major group consisted of 12 accessions, CM-14 genotype which is diverse form other genotypes grouped under cluster B. Cluster B again sub divided into 6 sub groups. The second cluster A consisted of 8 accessions separated at 3 linkage distance and divided into 2 groups. The genetic dissimilarity matrix based on Squared Euclidean Distance, showed a maximum dissimilarity (10%) between the genotypes Bangalore local-2 and Magadi local and the minimum dissimilarity (1%) between CO-2 and Solan Badam, CO-2 and Bangalore local-1, KIC-1 and IIHR-5. This analysis showed the potentiality of CM-14 variety due to its less vine length, less number of days to female flower appearance and early maturity as probable parent in hybridization programme.

Keywords


Cluster Analysis, CTAB, Dissimilarity Matrix, Genetic Diversity, RAPD.

References