Asian Journal of Bio Science

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Isolation of β-Glucosidase from Different Parts of Maize (Zea mays) and its Specific Activity Estimation


Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), India
     

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β-Glucosidase is a remarkable enzyme in the field of enzyme technology. Firstly, it helps in producing ethanol from the plant waste and subsequently helps to cope up the oil crisis as well as in alcohol based industries. Secondly, it improves the taste and aroma of wine, tea and fruits. In the present study β-Glucosidase was isolated from different plant parts of maize (Zea mays) and specific activity was estimated. The maximum crude enzyme was found in coleoptile (2.86ml) and maximum specific activity was estimated in thumb leaf (lower most leaf) as 43.446 whereas maximum protein was found in second leaf blade (9.241 mg/ml).

Keywords

Maize, β-Glucosidase, Specific activity, Protein.
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  • Isolation of β-Glucosidase from Different Parts of Maize (Zea mays) and its Specific Activity Estimation

Abstract Views: 285  |  PDF Views: 2

Authors

Santosh Kumar
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), India
O. P. Verma
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), India
Amit Alexander Charan
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), India

Abstract


β-Glucosidase is a remarkable enzyme in the field of enzyme technology. Firstly, it helps in producing ethanol from the plant waste and subsequently helps to cope up the oil crisis as well as in alcohol based industries. Secondly, it improves the taste and aroma of wine, tea and fruits. In the present study β-Glucosidase was isolated from different plant parts of maize (Zea mays) and specific activity was estimated. The maximum crude enzyme was found in coleoptile (2.86ml) and maximum specific activity was estimated in thumb leaf (lower most leaf) as 43.446 whereas maximum protein was found in second leaf blade (9.241 mg/ml).

Keywords


Maize, β-Glucosidase, Specific activity, Protein.