Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Bioefficacy of Mycopathogens Verticillium lecanii Zimmermen and Metarhizium anisopliae Metchnikoff against Sucking Pests of Bt Cotton


Affiliations
1 Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
     

   Subscribe/Renew Journal


Entomopathogenic fungi Verticillium lecanii Zimmermen and Metarhizium anisopliae Metchnikoff have generated a great deal of interest in recent years because of their potential as bio-control component in integrated pest management of cotton. These two mycopathogens were evaluated against sucking pests of Bt cotton during Kharif 2011 and 2012 at Cotton Improvement Project, Mahatma Phule Krishi Vidyapeeth, Rahuri to find out the scope and potentiality of V. lecanii and M.anisopliae against sucking pests of Bt cotton in intra hirsutum Bt hybrid (RCH-2Bt). Mycopathogens V.lecanii (2 ×108 cfu/gm and 2 ×1012 cfu/g) and M.anisopliae @ 2000 g/hectare were evaluated alone and in combination (V. lecanii + M.anisopliae). The observations were recorded on 3,5 and 10 days interval after each spray application. Pooled analysis of two years data revealed that V. lecanii (2 ×1012 cfu/g) @ 2000 g/hectare + M. anisopliae (2 ×1012 cfu/g) @ 2000 g/ha in combination was found more effective and significantly superior to its lower dose V. lecanii (2 ×108 cfu/g)@ 2000 g/ha + M. anisopliae (2 ×108 cfu/g) @ 2000 g/ha. It was proved superior over all other treatments in reducing aphids, jassids, thrips and whitefly population, however, it was at par with V. lecanii (2 ×1012 cfu/g) @ 2000 g/ha. Thus, V. lecanii (2 ×1012 cfu/g) @ 2000 g/ha was found to be the effective dose against all major sucking pests of cotton. Investigation on evaluation of entomopathogenic fungi against natural enemies of sucking pests in Bt cotton revealed that there was no significant adverse impact of V. lecanii and M. anisopliae on the activity of natural enemies viz., Chrysoperla and Coccinellids; when compared with the population of natural enemies in control. Sole and combine application of V. lecanii and M. anisopliae at all the evaluated doses were not found toxic to natural enemies.

Keywords

Bt Cotton, Kharif, Sucking Pests, Mycopathogen, Entomopathogenic Fungi, Verticillium lecanii, Metarhizium anisopliae.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Agarwal, G.P. (1990). Entomopathogenous fungi in India and management of insect pests. Indian Phytopathol., 34 : 131-142.

  • Ahmed, S.R., Ramm, G. and Faltin, G. (1984). Some salient features of the time-averaged ground vehicle wake. SAE Technical Paper Series 840 300, Detroit MI.

  • Balasubramanian, M. (1985). Integrated pest and disease management the need of the day : pest and disease managementoilseed, Pulses, Millets and Cotton, Tamil Nadu Agricultural Univ., Coimbatore, pp. 243-248.

  • Gopalkrishnan, C. and Narayanan, K. (1998). Occurrence of two entomofungal pathogens Verticillium lecanii (Zimmermen) and Metarhizium anisopliae (Metschnikoff) Sorokin on insect pests of cotton. Curr. Sci., 58: 867-868.

  • Isman, H.W. (1984). Evaluation of Entomopathogenic fungi, Verticillium lecanii and Metarhizium anisopliae for the control of sucking pest of cotton. J. Biol. Control., 19 (2) :19-22.

  • Isman, M.B., Wan, A.J. and Passreiter, C.M. (2001). In-secticidal activity of essential oils to the tobacco cut-worm, Spodoptera litura. Fitoterapia 72 : 65-68.

  • Kershaw, M.J., Moorhouse, E.R., Bateman, R.P., Reynolds, S.E. and Charnley, A.R. (1999). The role of destruxins in the pathogenicity of Metarhizium anisopliae for three species of insects. J. Invertebr. Pathol., 74 : 213-223.

  • Nahar, P., Yadav, P., Kulye, M., Hadappad, A., Hassani, M., Tuor, U., Keller, S., Chandele, A.G., Thomas, B. and Deshpande, M.V. (2004). Evaluation of indigenous fungal isolates, Metarhizium anisopliae M34412, Beauveria bassiana B3301 and Nomuraea rileyi N812 for the control of Helicoverpa armigera (Hubner) in pigeonpea field. J. Biol. Control., 18(1) : 18.

  • Perry, G E., Arnason, K.D. and Avato, E.W. (1998). Verticillium lecanii andMetarhizium anisopliae : a tool for biological control of sap sucking insects in cotton. J. Econ. Entomol., 38 :179-181.

  • Pray, C.E., Huang, J.D. and Qiao, F. (2001). Impact of Bt cotton in China.World Dev., 29 : 813-825.

  • Rachappa, V., Lingappa, S., Patil, R.K. and Kulkarni, K.A. (2004).Metarhizium anisopliae (Metsch.) Sorokin: a biorational IPM component for the managements of cotton pests. pp.103-110.

  • Satpute, U.S., Patil, V.N., Katole, S.R., Men, V.B., Bhagwat, V.R. and Thakare, A.V. (1990).Avoidable field losses due to sucking pests and bollworms in cotton. J. Appl. Zool. Res., 1(2) : 67-72.

  • Singha, D., Singha, B. and Dutta, B.K. (2006). Virulence of isolates of Metarhizium anisopliae, Beauveria bassiana (Ballsamo) vuillemin on tea termite. Indian J. Entomol., 68(1): 66-70.

  • Yussefi, M. and Willer, H. (2003). The world of organic agriculture: Statistics and future prospects, International Federation of Organic Agriculture Movements (IFOAM), Tholey-Theley.


Abstract Views: 376

PDF Views: 2




  • Bioefficacy of Mycopathogens Verticillium lecanii Zimmermen and Metarhizium anisopliae Metchnikoff against Sucking Pests of Bt Cotton

Abstract Views: 376  |  PDF Views: 2

Authors

U. B. Hole
Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
S. M. Gangurde
Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
N. D. Sarode
Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
R. W. Bharud
Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India

Abstract


Entomopathogenic fungi Verticillium lecanii Zimmermen and Metarhizium anisopliae Metchnikoff have generated a great deal of interest in recent years because of their potential as bio-control component in integrated pest management of cotton. These two mycopathogens were evaluated against sucking pests of Bt cotton during Kharif 2011 and 2012 at Cotton Improvement Project, Mahatma Phule Krishi Vidyapeeth, Rahuri to find out the scope and potentiality of V. lecanii and M.anisopliae against sucking pests of Bt cotton in intra hirsutum Bt hybrid (RCH-2Bt). Mycopathogens V.lecanii (2 ×108 cfu/gm and 2 ×1012 cfu/g) and M.anisopliae @ 2000 g/hectare were evaluated alone and in combination (V. lecanii + M.anisopliae). The observations were recorded on 3,5 and 10 days interval after each spray application. Pooled analysis of two years data revealed that V. lecanii (2 ×1012 cfu/g) @ 2000 g/hectare + M. anisopliae (2 ×1012 cfu/g) @ 2000 g/ha in combination was found more effective and significantly superior to its lower dose V. lecanii (2 ×108 cfu/g)@ 2000 g/ha + M. anisopliae (2 ×108 cfu/g) @ 2000 g/ha. It was proved superior over all other treatments in reducing aphids, jassids, thrips and whitefly population, however, it was at par with V. lecanii (2 ×1012 cfu/g) @ 2000 g/ha. Thus, V. lecanii (2 ×1012 cfu/g) @ 2000 g/ha was found to be the effective dose against all major sucking pests of cotton. Investigation on evaluation of entomopathogenic fungi against natural enemies of sucking pests in Bt cotton revealed that there was no significant adverse impact of V. lecanii and M. anisopliae on the activity of natural enemies viz., Chrysoperla and Coccinellids; when compared with the population of natural enemies in control. Sole and combine application of V. lecanii and M. anisopliae at all the evaluated doses were not found toxic to natural enemies.

Keywords


Bt Cotton, Kharif, Sucking Pests, Mycopathogen, Entomopathogenic Fungi, Verticillium lecanii, Metarhizium anisopliae.

References