Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Phytochemical Content, Antioxidant Activity and Reducing Power of Five Ethnic Medicinal Plants of Manipur


Affiliations
1 Department of Food Science and Nutrition, College of Home Science, Assam Agricultural University, Jorhat (Assam), India
     

   Subscribe/Renew Journal


Phytochemical content, antioxidant activity and reducing power of five ethnic medicinal plants of Manipur was analysed to study the antioxidant content commonly used by the traditional healers for the treatment of different inflammatory diseases and ailments. Antioxidant activity of methanolic extracts of five medicinal plants was investigated by using DPPH method which ranged from 47.82±0.041 per cent to 72.62±0.08 per cent inhibition. The phytochemical contents like total alkaloids, total flavonoids, total phenol, total carotenoids content ranged from 5.95±0.01 to 16.11±0.01 mg caffeine/100g on dry weight, 34.95±0.02 to 228.15±0.02 mg quercetin (QE)/100g on dry weight, 88.46±0.01 to 225.50±0.01 mg catechol equivalents (CE)/100g on dry weight, 0.81±0.005 to 3.80±0.005 mg/100g, respectively. Pearson correlation revealed a positive correlation between total phenol content, total flavonoids content, total carotenoids content and free radical scavenging activity (DPPH) of five medicinal plants extracts. However a negative correlation was found between total alkaloids content and free radical scavenging activity of medicinal plants extracts. The reducing power five medicinal plants extracts was statistically significant and positively correlated with DPPH free radical scavenging activity (r = 0.651; p<0.01). The study revealed that the plants with higher antioxidant activity (DPPH) showed high absorbance. Higher absorbance indicates more reducing power.

Keywords

Phytochemicals, Free Radicals, Antioxidant, Reducing Power, Medicinal Plants.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Aiyegroro, O.A. and Okoh, A.I. (2010). Preliminary phytochemical screening and in vitro antioxidant activities of aqueous extract of Helichrysum longifolium DC. BMC compl. And Alt. Med., 10:21.
  • Amic, D., Amic, D.B. and Trinajstic, N. (2003). Structure related scavenging activity relationships of flavanoids. Croat. Chem., 76: 55-61.
  • Anaya, A.L. (1999). Allopathy as a tool in the management of biotic resources in agroecosystems, Crit. Rev. Plant Sci.,18: 697-739.
  • Arunkumar, S. and Muthuselvam (2009). Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens.World J. Agril. Sc., 5 (5):572-576.
  • Chanda, S., Dave, R., Kaneria, M. and Nagani, K. (2009). Seaweeds: a novel, untapped source of drugs from sea to combat infectious diseases. Curr. Res. Technol. Edn. Top. Appl. Microb. Biotechnol., pp. 473-480.
  • Chung, K.T., Lu, Z. and Chou, M.W. (2002). Mechanism of inhibition of tannic acid and related compounds on the growth of intestinal bacteria. Food Chem. Toxicol., 36: 1053-1060.
  • Dewick, P.M. (1996). Tumor inhibition from plants : Tease and Evans. In : Plants With Antiprotozoal Activity. 14th edn. Phillipson, J.D. and Wright, C.W. (eds.). Tease and Evans, Pharmacognosy, WB Saunders Company, London., pp. 6-12.
  • Edeoga, H.O., Okwu, D.E. and Mbaebie, B.O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African J. Biotechnol., 4(7): 685-688.
  • Fiedor, J., Fiedor, L., Haessner, R. and Scheer, H. (2005). Cyclic endoperoxides of -carotene, potential pro-oxidants, as products of chemical quenching of singlet oxygen. Biochimica et Biophysica Acta., 1709: 1-4.
  • Huang, S. W., Frankel, E. N., Schwarz, K., Aeschbach, R. and German, J.B. (1996). Antioxidant activity of carnosic acid and methylcarnosate in bulk oils and oil-in-water emulsions. J. Agric. Food Chem.,44: 2951-2956.
  • Kumar, K. and Abbas, S.G. (2012). Ethnomedicinal composition depends on floristic composition: A case studied in Sal forests of Jharkhand. Internat. J. Pharmacy & Life Sci., 3 (5) : 1710-1719.
  • Li, B.B., Smith, B. and Hossain, M.M. (2006). Extraction of phenolics from citrus peels: I. Solvent extraction method. Separ. Purif. Technol., 48: 182-188.
  • Lucia, R., Magdalena, M., Daniela, K. and Milan, S. (2004). Antiradical and antioxidant activities of alkaloids from Mahonia aquifolium. Structural aspects, Bioorg. Med. Chem., 12 (17): 4709-4715.
  • Malik, C.P. and Singh, M.B. (1980). In: Plant Enzymology and histo Enzymology. Kalyani Publishers New Delhi, p. 286.
  • Oyaizu, M. (1986). Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr., 7 : 307-15.
  • Pietta, P.G. (2000). Flavonoids as antioxidants. J. Nat. Products., 63: 1035–1042.
  • Rahman, K. (2007). Studies on free radicals, antioxidants, and cofactors, Clin Interv Aging., 2: 219-236.
  • Schroeter, H., Boyd, C., Spencer, J.P.E., Williams, R.J., Cadenas, E. and Rice-Evans, C. (2002). MAPK signaling in neurodegeneration: Influences of flavonoids and of nitric oxide. Neurobiol. Aging., 23 : 861-880.
  • Seifu, T. (2012). Ethnobotanical and Ethnopharmaceutical Studies on Medicinal Plants of Chifra District, Afar Region, North Eastern Ethiopia. M.Sc. Thesis, Addis Ababa University, Addis Ababa, Ethiopia.
  • Shamsa, F., Hamidreza, M., Rouhollah, G. and Mohammadreza, V. (2008). Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J. Pharm. Sci., 32: 17-20.
  • Sheik, S. and Chandrashekar, K.R. (2014). Antimicrobial and antioxidant activities of Kingiodendron pinnatum (DC.) Harms and Humboldtia brunonis Wallich: endemic plants of the Western Ghats of India. J. Natn. Sci. Foundation Sri Lanka., 42(4): 307-313.
  • Shimada, K., Fujikawa, K., Yahara, K. and Nakamura, T. (1992). Antioxidative properties of xanthone on the auto oxidation of soybean in cylcodextrin emulsion. J. Agril. Food Chem., 40: 945-948.
  • Soni, A. and Sosa, S. (2013). Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. J. Pharm. Phytochem., 2 (4): 22-29.
  • Tanab, H., Yoshida, M. and Tomita, N. (2002). Comparison of the antioxidant activities of 22 commonly used herbs and spices on the lipid oxidation of pork meat. J. Animal Sci., 73: 389-393.
  • Vani, T., Rajani, M., Sarkar, S. and Shishoo, C.J. (1997). Antioxidant properties of the ayurvedic formulation, Triphala and its constituents. Inter. J. Pharmac., 35: 313-317.
  • Willcox, M.L. and Bodeker, G. (2004). Traditional herbal medicines for malaria. Clin. Rev. British Med. J., 329: 1156–1159.

Abstract Views: 290

PDF Views: 0




  • Phytochemical Content, Antioxidant Activity and Reducing Power of Five Ethnic Medicinal Plants of Manipur

Abstract Views: 290  |  PDF Views: 0

Authors

Okram Abemsana Devi
Department of Food Science and Nutrition, College of Home Science, Assam Agricultural University, Jorhat (Assam), India
Mamoni Das
Department of Food Science and Nutrition, College of Home Science, Assam Agricultural University, Jorhat (Assam), India
Ananta Saikia
Department of Food Science and Nutrition, College of Home Science, Assam Agricultural University, Jorhat (Assam), India
Pranati Das
Department of Food Science and Nutrition, College of Home Science, Assam Agricultural University, Jorhat (Assam), India

Abstract


Phytochemical content, antioxidant activity and reducing power of five ethnic medicinal plants of Manipur was analysed to study the antioxidant content commonly used by the traditional healers for the treatment of different inflammatory diseases and ailments. Antioxidant activity of methanolic extracts of five medicinal plants was investigated by using DPPH method which ranged from 47.82±0.041 per cent to 72.62±0.08 per cent inhibition. The phytochemical contents like total alkaloids, total flavonoids, total phenol, total carotenoids content ranged from 5.95±0.01 to 16.11±0.01 mg caffeine/100g on dry weight, 34.95±0.02 to 228.15±0.02 mg quercetin (QE)/100g on dry weight, 88.46±0.01 to 225.50±0.01 mg catechol equivalents (CE)/100g on dry weight, 0.81±0.005 to 3.80±0.005 mg/100g, respectively. Pearson correlation revealed a positive correlation between total phenol content, total flavonoids content, total carotenoids content and free radical scavenging activity (DPPH) of five medicinal plants extracts. However a negative correlation was found between total alkaloids content and free radical scavenging activity of medicinal plants extracts. The reducing power five medicinal plants extracts was statistically significant and positively correlated with DPPH free radical scavenging activity (r = 0.651; p<0.01). The study revealed that the plants with higher antioxidant activity (DPPH) showed high absorbance. Higher absorbance indicates more reducing power.

Keywords


Phytochemicals, Free Radicals, Antioxidant, Reducing Power, Medicinal Plants.

References