High Performance Thin Layer Chromatography Method for Simultaneous Estimation of Cefepime Hydrochloride and Sulbactam Sodium

A Simple High Performance Thin Layer Chromatography method for the simultaneous estimation of cefepime hydrochloride and sulbactam sodium was developed. The determination was carried on Silica Gel 60 GF254 HPTLC Plates using the mobile phase of chloroform:ethyl alcohol: Diethyl amine: water (12:7:1:0.4V/V). The absorbances of the spots were measured by densitometry at 254nm. The Retention Factor (Rf) was found to be 0.17 for cefepime hydrochloride and 0.76 for sulbactam sodium respectively. Cefepime hydrochloride and sulbactam sodium showed linear response at concentration range 4-20 μg/band and 2-10 μg/band repepectively. The Correlation co-efficient (r²) for cefepime hydrochloride and sulbactam sodium was found to be 0.9997 and 0.9997 respectively. The percentage recoveries obtained for cefepime hydrochloride and sulbactam sodium in range form 99.87-100.12 and 98.91-100.50.