Simultaneous Determination of Saxagliptin Hydrochloride and Glibenclamide in Synthetic Mixture Using Spectrophotometric Technique (First Order Derivative Method)

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Saxagliptin Hydrochloride and Glibenclamide in synthetic mixture using first order derivative zero-crossing method. Saxagliptin Hydrochloride showed zero crossing point at 315.00nm while Glibenclamide showed zero crossing point at 229.40nm. The dA/dλ was measured at 229.40nm for Saxagliptin Hydrochloride and 315.00nm for Glibenclamide and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.9995) in the range of 5-25μg/ml for Saxagliptin Hydrochloride at 229.40nm. The linear correlation was obtained (r2>0.9994) in the range of 5-25 μg/ml for Glibenclamide at 315.00nm. The limit of determination was 0.243μg/ml and 0.317μg/ml for Saxagliptin Hydrochloride and Glibenclamide, respectively. The limit of quantification was 0.738μg/ml and 0.960μg/ml for Saxagliptin Hydrochloride and Glibenclamide respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in binary mixture.