Estimation of Andrographolide Content in Aqueous Extract of Siddha Formulations by HPTLC

Indigenous system of Siddha medicine is playing a vital role in the prevention of viral infections such as dengue fever, chikungunya and swine flu. The main aim of the study was to estimate the andrographolide content in aqueous extracts of Siddha formulations containing Andrographis paniculata supplied in Tamilnadu for prevention of dengue fever, chikungunya and swine flu. HPTLC method was adopted for determining the content of and rographolide in Siddha formulations using and rographolide as marker compound. The HPTLC method was performed using HPTLC aluminium sheets precoated with silica gel 60 GF₂₅₄ as stationary phase and ethyl acetate: n-hexane (8.5:1.5 v/v) as the mobile phase. The developed chromatogram was scanned at 233 nm using Camag scanner III. The R_f value of standard and rographolide and andrographolide in the samples were found to be in the range of 0.31 to 0.33.Siddha formulation collected from Siddha VaidhyaNilayam (Dharmapuri Siddha Traditional Formulation) showed more content of andrographolide compared to other Sidhha formulations.