Development and Validation of Simultaneous Estimation of Two Catecholamines in Combine Dosage Form by HPTLC Method

Multi component dosage forms are to be effective due to their combined mode of action in the body and Quantitative analysis of such Combination of drugs is an important tool in an industry. Combination of two catecholamines, levodopa and carbidopa has been used in combination for the treatment of Parkinson disease. HPTLC method developed for quantitative estimation of levodopa and carbidopa in their combined dosage form by using different solvent system. The developed method consisted of Acetone: Chloroform: n-butanol: GAA: water (5 : 5 : 4.0 : 3.5 : 2.0, v/v/v/v/v) as mobile phase. Saturation time was kept 30 minutes with run length of 60 mm. the drugs were separated at the Rf value of 0.26 for levodopa and 0.83 for carbidopa. By this method also analysed and separated a degradation product of the levodopa, carbidopa and mixture of both in solution mixture. So If degradation done during storage or in stability study, easily determined the degraded products by using this method. In industry it is useful degraded stability study. The developed HPLC method with solvent system found simple, rapid, robust, accurate and most sensitive method for determination of bcombined dosage form of catecholamines hence best method to utilize at industry level.