Development of UV Spectroscopy Fingerprinting Method of Total Polyphenols as Tannic Acid in Manibhadra yoga

Ayurvedic formulation is essential in order to assess the quality of drugs, based on the concentration of their active principles. The main objective of the present study is to develop a simple, rapid and validated UV fingerprint method for estimation of total polyphenols as tannic acid in Manibhadra yoga and crude drugs. Tannic acid is one of the major constituent of Manibhadra yoga and it is official in Ayurvedic formulary of India and it is the most common formulation used for respiratory disorders in Ayurvedic medicinal preparation. It content of the well documented medicinal important plants Embelia ribe (Vidanga), Embelica officinalis (Amalaki), Terminalia chebula (Haritaki), Operculina turpethum (Nishotha) and guda (jeggary). The detection wavelength of tannic acid was 277.2 nm. This method is used for the estimation of tannic acid in formulations and identifies and assessed its quality by using UV method. The estimation was carried out with three laboratory batches and one marketed formulation.