The Estimation of Cefquinome Sulphate in Suspension Form by RP-HPLC

A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Cefquinome Sulphate in suspension form. An XTerra(R) analytical coloumn (C18, 250 x 4.6 mm, 5 μm particle size), with mobile phase consisting of mixture of buffer 0.02M Ammonium Acetate in water and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 234 nm. The retention time was 6.06 min. The detector response was linear in the concentration of 4 - 48 mcg/mL. The respective linear regression equation being y=849408x-849408. The limit of detection and limit of quantification was 0.01mcg/mL and 0.03mcg/mL respectively. The percentage assay of Cefquinome Sulphate was 99.4%. The method was validated by determining its accuracy, precision and system suitability.

The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Cefquinome Sulphate in bulk drug and in its suspension form.