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Quantitative Evaluation of Carbohydrate Levels in Different Natural Foodstuffs by UV-Visible Spectrophometer


Affiliations
1 Sri Indu Instituite of Pharmacy, Sheiguda, R.R dist. 501510, India
2 Venkateshwara Institute of Pharmaceutical Sciences, Chelapally, Nalgonda 508002, India
     

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A rapid method was developed for the quantitative estimation of carbohydrates present in the different natural foodstuffs by UV-visible spectrophotometer. The sample extract of the raw rice, green gram, black gram, ground nut, guava, grape, banana, carrot, bean and milk were subjected by using anthrone as reagent for the quantitative estimation of the carbohydrate. These samples absorbance was read in UV-visible spectrophotometer at the wavelength of 750nm. The recommendation for the general population is that carbohydrate should supply 50 to 55 percent of total calories, and 130 grams per day (520 calories per day) for male and female adults and for athletes is between 55 and 65 percent of total calories.

Keywords

Food Stuffs, UV-Visible Spectrophotometer, Wavelength, Anthrone, Absorbance.
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  • Quantitative Evaluation of Carbohydrate Levels in Different Natural Foodstuffs by UV-Visible Spectrophometer

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Authors

M. Sravan Prasad
Sri Indu Instituite of Pharmacy, Sheiguda, R.R dist. 501510, India
Madhu
Sri Indu Instituite of Pharmacy, Sheiguda, R.R dist. 501510, India
G. Venkateshwalu
Venkateshwara Institute of Pharmaceutical Sciences, Chelapally, Nalgonda 508002, India
Manoranjan Sabath
Venkateshwara Institute of Pharmaceutical Sciences, Chelapally, Nalgonda 508002, India

Abstract


A rapid method was developed for the quantitative estimation of carbohydrates present in the different natural foodstuffs by UV-visible spectrophotometer. The sample extract of the raw rice, green gram, black gram, ground nut, guava, grape, banana, carrot, bean and milk were subjected by using anthrone as reagent for the quantitative estimation of the carbohydrate. These samples absorbance was read in UV-visible spectrophotometer at the wavelength of 750nm. The recommendation for the general population is that carbohydrate should supply 50 to 55 percent of total calories, and 130 grams per day (520 calories per day) for male and female adults and for athletes is between 55 and 65 percent of total calories.

Keywords


Food Stuffs, UV-Visible Spectrophotometer, Wavelength, Anthrone, Absorbance.