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Development and Validation of UV Spectrophotometric Method of Darifenacin Hydrobromide in Bulk and Tablet Dosage Form


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1 Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, India
     

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A simple, accurate and specific UV spectrophotometric method was developed and validated for the estimation of Darifenacin hydrobromide in bulk and tablet dosage form. Analyte showed the absorption maxima in 0.1M Hydrochloric acid at 284nm. The percentage recovery of Darifenacin Hydrobromide was 99.69±0.14%. The developed method was validated with respect to linearity, accuracy, precision and specificity. Beer's law was obeyed in the concentration range of 20.00μg/mL to 46.50μg/mL, having line equation y=0.059x+0.0177 with correlation coefficient of 0.999. Results of the analysis were validated statistically as per ICH guidelines.

Keywords

UV Spectrophotometry, Darifenacin, Hydrobromide, Estimation, Tablets.
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  • Development and Validation of UV Spectrophotometric Method of Darifenacin Hydrobromide in Bulk and Tablet Dosage Form

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Authors

D. Sridharan
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, India
Umarani A. Thenmozhi
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, India
L. Pavan Kumar
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, India
Aswani Dutt Chintalapati
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, India
M. Venkata Ramanaiah
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, India
Yelika Phanikishore
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, India

Abstract


A simple, accurate and specific UV spectrophotometric method was developed and validated for the estimation of Darifenacin hydrobromide in bulk and tablet dosage form. Analyte showed the absorption maxima in 0.1M Hydrochloric acid at 284nm. The percentage recovery of Darifenacin Hydrobromide was 99.69±0.14%. The developed method was validated with respect to linearity, accuracy, precision and specificity. Beer's law was obeyed in the concentration range of 20.00μg/mL to 46.50μg/mL, having line equation y=0.059x+0.0177 with correlation coefficient of 0.999. Results of the analysis were validated statistically as per ICH guidelines.

Keywords


UV Spectrophotometry, Darifenacin, Hydrobromide, Estimation, Tablets.