Asian Journal of Pharmaceutical Analysis

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Capillary Electrophoresis: A Potential Tool for Separation and Analysis-Brief Review


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1 Govt. College of Pharmacy, Osmanpura, Aurangabad 431 005, (M.S.), India
     

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Capillary electrophoresis (CE) is a potential analytical and separation technique for wide variety of analytes, ranging from micro to macromolecules of biological or chemical origin belongs to either inorganic or organic class possesses simple or complex molecules. CE technology has been valuable for the comprehensive determination of drug and process related impurities. CE is a family of electrokinetic separation methods performed in capillaries and in micro-fluidic channels rely on a high voltage electric field being applied over a solution which is held in a capillary tube. CE classified according to mode of separation on the basis of differences in charge, size and frictional force, offers fast separations with excellent efficiency. CE is one of favorite analytical tool for assay of pharmaceutical API including determination of drug related impurities, as it provides reliable result in minimum time period; also, it offers a high degree of automation and requires minimal sample preparation and. CE possess other versatile applications such as chiral, stoichiometric and bioanalysis of pharmaceutical API. The current review presents basic principles underlying different modes of CE, and their application in analysis of pharmaceutical analysis.

Keywords

Capillary Electrophoresis, Non Aqueous Capillary Electrophoresis, Capillary Gel Electrophoresis, Capillary Isoelectric Focusing, Capillary Isotachophoresis.
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  • Harry Whatley, Basic Principles and Modes of Capillary Electrophoresis, Clinical and Forensic Applications of Capillary Electrophoresis:21-22

  • European Pharmacopoeia 5.0, 2.2.47. Capillary electrophoresis: 74

  • Yan Xu, Tutorial Capillary Electrophoresis. The Chemical Educator, 1996; 1(2):1-5.

  • Freitag R, Capillary Gel Electrophoresis, Electrophoresis, Academic Press, Switzerland, 2000, II:1201-1207

  • Ohnesorge J, Neusüb C, Watzig H. Quantization in capillary electrophoresis-mass spectrometry. Electrophoresis 2005; 26:3973-3987.

  • Pereira M, Lai EPC. Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay, J Nanobiotechnol 2008;6:10 doi:10.1186/1477-3155-6-10

  • Solangi1 AR, Memon SQ, Khuhawar MY, Bhanger MI. Quantitative analysis of eight cephalosporin antibiotics in pharmaceutical products and urine by capillary zone electrophoresis, ActaChromatographica, 2007; 19:81-96.

  • Wang F, Khaledi MG. Chiral separations by non-aqueous capillary electrophoresis, Anal Chem 1996; 68(19): 3460-3467.

  • Maria SilvanaBellini, ZdenekDeyl, Ivan Miksık. Non-aqueous capillary electrophoresis. Application to the separation of complex mixtures of organic acids by ion-pairing mechanism, Forensic Science International. 1998; 92:185-199.

  • Cevdet DEM, IR, Yasin YUCEL. Optimization of the Non-aqueous Capillary Electrophoresis Separation of Metal Ions Using Mixture Design and Response Surface Methods, Turk J Chem, 2006; 30:181-192.

  • Erim FB. From capillary electrophoresis to capillary electrochromatography: An overview. Hacettepe J Biol and Chem 2009; 37(2):77-82.

  • Gatschelhofer C, Schmid MG, Schreiner K, Pieber TR, SinnerFM, Gubitz GJ. Biochem. Biophys. Methods, 2009; 69:67-77.

  • Ivan MikDk, PavlaSedlkov. Capillary electro-chromatography of proteins and peptides, J. Sep. Sci., 2007; 30:1686-1703.

  • Righetti PG, GelfiCapillary C. Iso electric focusing, Electrophoresis, Academic Press, Switzerland, 2000, II:1208-1215.

  • SahdeckaH J and PolonskyH J. Capillary Isotachophoresis, Electrophoresis, Academic Press, Switzerland. 2000; II:1215-1222.

  • Li SFY. Capillary electrophoresis: Principles, practice, and applications. Elsevier Science Publishers: The Netherlands, 1992.

  • Shigeru Terabe. Micellarelectrokintic chromatography. Humana Press. 125-155. DOI 10.1385/0-89603-315-5:125

  • Terabe S, Matsubara N, Ishihama Y, Okada Y. Micro-emulsionelectrokinetic chromatography: Comparison with miceller-electrokinetic chromatography. J Chromatogr 1992;608:23-29.

  • Mahuzier PE, Aurora MS, PradoBJ, ClarkERM, Kedor-Hackmann, Altria KD. An Introduction to the Theory and Application of Micro-emulsionElectrokinetic Chromatography. 2003; CE Currents,

  • Wu Y, Wang Y, Zhao Z, Wu K, Wang Y, Yan C. Pressurized capillary electro-chromatography: Instrumentation, columns, and applications. Amer Lab 2010; 42(2):38-41.

  • Krull IS, Stevenson RL, Misty K, Swartz ME, Capillary electro-chromatography and pressurized flow capillary electrochromatography, HNB Publishing, New York, 2000, 240.

  • Lin ZA, Wu XP, Lin XC, Xie ZH. End-column chemi-luminescence detection for pressurized capillary electro-chromatographic analysis of norepinephrine and epinephrine. J Chromatogr A 2007;1170:118–121.

  • Qu QS, Mangelings D, Shen F, Hua XY, Yan C, Zhang YK, et al. Pressurized capillary electro-chromatographic assay of trimethoprim impurities using 1 microm particle-based columns. J Chromatogr A 2007; 1169:228-234.

  • Frank A. Gomez, Jose Zavaleta, Dinora Chinchilla, Abby Brown, Alejandra Ramirez, Violet Calderon, Taguhi Sogomonyan, Recent Developments in Affinity Capillary Electrophoresis: A Review, Current Analytical Chemistry, 2006, :1-8.

  • Schiewe J. Principles of capilliary electrophoresis. In:Affinity capillary electrophoresis in pharmaceutics and biopharmaceutics. Neubert RHH, Ruttinger HH (eds.), Marcel Dekker Inc. New York, 2003.

  • Wang C, Oleschuk R, Ouchen F, Li J, Thibault P, Harrison DJ. Integration of immobilized trypsin bead beds for protein digestion within a microfluidic chip incorporating capillary electrophoresis separations and an electrospray mass spectrometry interface. Rapid Commun. Mass SP. 2000;14:1377-1383.

  • Victor M. Ugaz and Jennifer L. Christensen, Electrophoresis in microfuidic system, Springer US 2007; 393-438. DOI 10.1007/978-0-387-68424-6_10

  • Michalska K, Pajchel G, Tyski S. Capillary electrophoresis method for simultaneous determination of penicillin G, procaine and dihydrostreptomycin in veterinary drugs. J. Chromatogr. B 2004; 800:203-209.

  • Pérez-Ruiz T, Martínez-Lozano C, Sanz A, Bravo E. Development and validation of a quantitative assay for raloxifene by capillary electrophoresis. J. Pharm. Biomed. Anal. 2004; 34891-897.

  • Jamali B. Lehmann, S. Development and validation of a highresolution capillary electrophoresis method for multi-analysis of ragaglitazar and arginine in Active Pharmaceutical Ingredients and low-dose tablets. J. Pharm. Biomed. Anal. 2004; 34:463-472.

  • KrcalK, Tuncel M, Aboul-Enein HY. Determination of pravastatin in tablets by capillary electrophoresis. Farmaco 2004;59:241-244.

  • VelikinacI, Cudina O, Jankovic I, Agbaba D, Vladimirov S. Comparison of capillary zone electrophoresis and high performance liquid chromatography methods for quantitative determination of ketoconazole in drug formulations. Farmaco 2004; 59:419-424.

  • LeenaSuntornsuk, Capillary Electrophoresis in Pharmaceutical Analysis:A Survey on Recent Applications, Journal of Chromatographic Science. October 2007; 45: 1-19.

  • Wang R, Jia Z-P,Fan J-J, Chen L-R, Xie H, Ma J, Ge X, Zhang Q, Ao Y, Wang J. CE with hydroxypropyl-β- cyclodextrin as chiral selector, for separation and determination of the enantiomers of amlodipine in the serum of hypertension patients. Chromatographia 65;2007:575–79.

  • Hedeland Y, Lehtinen J, Petterson C. Ketopinic acid and di-isoproylideneketogulonic acid as chiral ion-pair selectors in capillary electrophoresis. J. Chromatogr. 2007; A1141:287-94.

  • Martínez-Gómez MA, Martínez-Pla JJ, Sagrado S, Villanueva-Camaña RM, Medina-Hernández MJ. Chiral separation of oxprenolol by affinity electrokinetic chromatography- partial filling technique using human serum albumin as chiral selector. J. Pharm. Biomed. Anal. 2005;39:76-81.

  • BlancoM, Valverde I. Chiral and non-chiral determination of Dopa by capillary electrophoresis J. Pharm. Biomed. Anal. 2003;31: 431-438.

  • KuoCY, WuHL, WuSM. Enantiomeric analysis of methotrexate in pharmaceuticals by cyclodextrin-modified capillary electrophoresis. Anal. Chim. Acta 2002;471:211-217.

  • Kevin Altria, Alex Marsh, CariSänger-van de Griend, Capillary electrophoresis for the analysis of small-molecule pharmaceuticals, Electrophoresis 2006; 27: 2263-2282.

  • Altria KD, Determination of drug-related impurities by capillary electrophoresis, Journal of Chromatography A, 1996;735:43-56.

  • Sabbah, S, Scriba, GK. Development and validation of a capillary electrophoresis assay for the determination of 3,4-diaminopyridine and 4-aminopyridine including related substances. J. Chromatogr. A 2001;907:321-328.

  • FabreH, PerrinC, Bosc N. Determination of homotaurine as impurity in calcium acamprosate by capillary zone electrophoresis. J. Chromatogr. A. 1999;853:421-430.

  • Swartz M, Liq J. Chromatogr., 14; 1991:923.

  • Gotti R, Furlanetto S, Andrisano V, Cavrini V, Pinzauti SJ. Chromatogr. A, 2000; 875:411-422.

  • Kodama S, Yamamoto A, Matsunaga A, Soga T, Minoura KJ. Chromatogr. A, 2000;875:371-377.

  • Ramseier A., Caslavaska J, Thormann W. electrophoresis, 1999; 20:2726-2738.

  • Ban E., Choi S., Lee J.A., Lho D.S., Yoo Y.S., J. Chromatogr. A, 1999; 853:439–447.

  • Mandrioli R, Pucci V, Sabbioni C, Bartoletti C, Fanali S, Raggi MA, J. Chromatogr. A 2004; 2004:253–260.

  • GluckSJ, Benko MH, HallbergRK, SteeleKP. Indirect determination of octanol-water partition coefficients by micro-emulsionelectrokinetic chromatography. J. Chromatogr. A 1996;744:141-146.

  • Yang, S, Bumgarner JG, Kruk LF, Khaledi MG. Quantitative structure-activity relationships studies with micellar-electrokinetic chromatography influence of surfactant type and mixed micelles on estimation of hydrophobicity and bioavailability. J. Chromatogr. A 1996;721:323-335.

  • IshihamaY, Oda Y, AsakawaN. A hydrophobicity scale based on the migration index from micro-emulsionelectrokinetic chromatography of anionic solutes Anal. Chem. 1996;68:1028-1032.

  • Wan H, Holmen A, Nagard M, Lindberg W. Rapid screening of pKa values of pharmaceuticals by pressure-assisted capillary electrophoresis combined with short-end injection. J. Chromatogr. A 2002;979:369-377.

  • Ornskov E, Linusson A, Folestad S. (2003) Determination of dissociation constants of labile drug compounds by capillary electrophoresis. J. Pharm. Biomed. Anal. 2003;33:379-391


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  • Capillary Electrophoresis: A Potential Tool for Separation and Analysis-Brief Review

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Authors

Rajesh B. Nawale
Govt. College of Pharmacy, Osmanpura, Aurangabad 431 005, (M.S.), India
Hrishikesh H. Rajput
Govt. College of Pharmacy, Osmanpura, Aurangabad 431 005, (M.S.), India
Mayura A. Kale
Govt. College of Pharmacy, Osmanpura, Aurangabad 431 005, (M.S.), India
Uday A. Deokate
Govt. College of Pharmacy, Osmanpura, Aurangabad 431 005, (M.S.), India

Abstract


Capillary electrophoresis (CE) is a potential analytical and separation technique for wide variety of analytes, ranging from micro to macromolecules of biological or chemical origin belongs to either inorganic or organic class possesses simple or complex molecules. CE technology has been valuable for the comprehensive determination of drug and process related impurities. CE is a family of electrokinetic separation methods performed in capillaries and in micro-fluidic channels rely on a high voltage electric field being applied over a solution which is held in a capillary tube. CE classified according to mode of separation on the basis of differences in charge, size and frictional force, offers fast separations with excellent efficiency. CE is one of favorite analytical tool for assay of pharmaceutical API including determination of drug related impurities, as it provides reliable result in minimum time period; also, it offers a high degree of automation and requires minimal sample preparation and. CE possess other versatile applications such as chiral, stoichiometric and bioanalysis of pharmaceutical API. The current review presents basic principles underlying different modes of CE, and their application in analysis of pharmaceutical analysis.

Keywords


Capillary Electrophoresis, Non Aqueous Capillary Electrophoresis, Capillary Gel Electrophoresis, Capillary Isoelectric Focusing, Capillary Isotachophoresis.

References