Asian Journal of Pharmaceutical Analysis

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Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Diazepam and Propranolol in Bulk Drug and its formulations


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1 University Institute of Pharmacy, Ravishankar Shukla University, Raipur (CG) India-492010, India
     

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A simple, efficient, precise and accurate Vierodt's method have been developed for the estimation of Diazepam and Propranolal in pure and in fixed dose Combination. In this method UV spectrum of Diazepam and Propranolal were overlained which involves the formation of Simultaneous equation at maximum wavelength 241 nm and 289 nm. Both the drugs obeyed Beer's law in the concentration range 2-25 μg/ml and 1-45 μg/ml for Diazepam and Propranolal , respectively. The accuracy of the method was determined by recovery studies and was found to be 100.14±0.219 % and 100.17±0.134 % for Diazepam and Propranolal , respectively. The method was validated as per ICH guidelines. The method was found to be simple, economic, accurate and reproducible and can be used for routine analysis of Diazepam and Propranolal in pure and in fixed dose combination.

Keywords

Vierodt’s Method, Diazepam, Propranolal, ICH Guidelines
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  • Pharmacopoeia of India. Controller of Publications, New Delhi. 1985; I: 162-163.

  • Murty SSN, Rao GR and Bajrangrao B. Colorimetric determination of diazepam hydrochloride. East. Pharm. 1981; 24(286): 121- 122.

  • Sane RT, Kamat RP, Gijare AS and Deshpande DS. A simple colorimetric method for the determination of diazepam hydrochloride from pharmaceutical preparation. Indian Drugs. 1983; 20 (8): 331-332.

  • Emmanuel J and Shetty AR. Colorimetric estimation of diazepam hydrochloride in pharmaceutical preparations. Indian Drugs. 1984; 21 (4): 167- 168.

  • Kamlapurkar OS and Kamat GJ. Colorimetric method for estimation of diazepam hydrochloride in its formulations. East. Pharm. 1984; 27(319): 137- 138.

  • Sivachandra Y, Rao SV and Ramamurthy PS. Determination of hostacycline and diazepam using thorium (IV) as spectrophotometric reagents. Indian J. Pharm. Sci.1996; 58(4): 157-158.

  • Chaturvedi SC, Jain S and Tiwari M. Propranolol HCldiazepam- simultaneous estimation by first order derivative spectroscopy. Indian Drugs. 1998; 35(11): 696-699.

  • Zurghi A, Kabriaeezadeh A and Ahmed K. Rapid high performance liquid chromatographic method for determination of diazepam in human plasma. Boll. Chin. Farm. 2001; 140: 112- 114, Through Int. Pharma. Abstract, 2002,3902862.

  • Pharmacopoeia of India. Controller of Publications, New Delhi. 1985; I: 425.

  • Shingbal DM and Prabhudesai JS. Spectrophotometric estimation of propranolol hydrochloride. Indian Drugs. 1984; 21(7): 304-305.

  • Rao GR, Raghuveer S and Khadgapathi P. Colorimetric determination of propranolol hydrochloride and its tablets using 3- methyl- 2- benzothiazolinone hydrazone hydrochloride. East. Pharm. 1987; 30(354): 145- 146.

  • Reddy MN, Gowrisankar D, Sreedhar K and Devalorao G. Extractive spectrophotometric determination of propranolol. East. Pharm. 1993; 36(428): 177- 178.

  • Erran SV and Tipnis HP. Simple spectrophotometric analysis of propranolol hydrochloride and hydrochlorothiazide from combined pharmaceutical dosages. Indian Drugs. 1994; 31(2): 65- 68.

  • Zhag JR, Pong H, Wang WH, Hpu Z and Zheng Q. Second order derivative spectrophotometric determination of propranolol hydrochloride tablets. Through Int. Pharma. Abstract. 1998; 3501028.

  • Sajjan AG, Seetharmappa J and Masti SP. Spectrophotometric determination of propranolol hydrochloride in pharmaceutical preparations. Indian J. Pharm. Sci. 2002; 64(1): 68-70.

  • Khalil S and El-Rabiehi MM. Indirect atomic absorption spectrometric determination of pindolol, propranolol and levamisole hydrochloride based on formation of ion associates with manganese thiocynate and potassium ferricyanide. J. Pharm. Biomed. Anal. 2000; 22(1): 7- 12.

  • Khalil S and Borham N. Indirect atomic absorption spectrometric determination of pindolol, propranolol and levamisole hydrochloride based on formation of ion associates with ammonium reinekate and sodium cobaltinitrite. J. Pharm. Biomed. Anal. 2000; 22(2): 235- 240.

  • El-Ries MA, Attia FM and Ibrahim SA. AAS and spectrophotometric determination of propranolol hydrochloride and metoprolol tartrate. J. Pharm. Biomed. Anal. 2001; 24(2): 179- 187.

  • Shinde VM, Desai BS and Tendolkar NM. Simultaneous determination of propranolol hydrochloride and hydrochlorothiazide in tablets by quantitative TLC. Indian Drugs. 1994; 31(5): 192- 196.

  • United State Pharmacopoeia. United States Pharmacopoeial Convention, Inc. 2006; 26: 1843.

  • Santoro MI, Cho HS and Kedor-Hackman ER. Enatiometric separation and quantitative determination of propranolol in tablets by chiral HPLC. Drug. Dev. Ind. Pharm. 2002; 27: 693- 697.

  • Pantullu ARR, Giridhar R, Anjaneyulu Y and Avadhanulu AB. Gas liquid chromatographic determination of propranolol and dicyclomine hydrochloride in single and combined dosage forms. East. Pharm. 1993; 36(432): 123- 125.

  • ICH Tripartite Guideline on Validation of Analytical Procedure, Text and methodology Q2 (R1), 2005.


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  • Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Diazepam and Propranolol in Bulk Drug and its formulations

Abstract Views: 570  |  PDF Views: 4

Authors

S. J. Daharwal
University Institute of Pharmacy, Ravishankar Shukla University, Raipur (CG) India-492010, India

Abstract


A simple, efficient, precise and accurate Vierodt's method have been developed for the estimation of Diazepam and Propranolal in pure and in fixed dose Combination. In this method UV spectrum of Diazepam and Propranolal were overlained which involves the formation of Simultaneous equation at maximum wavelength 241 nm and 289 nm. Both the drugs obeyed Beer's law in the concentration range 2-25 μg/ml and 1-45 μg/ml for Diazepam and Propranolal , respectively. The accuracy of the method was determined by recovery studies and was found to be 100.14±0.219 % and 100.17±0.134 % for Diazepam and Propranolal , respectively. The method was validated as per ICH guidelines. The method was found to be simple, economic, accurate and reproducible and can be used for routine analysis of Diazepam and Propranolal in pure and in fixed dose combination.

Keywords


Vierodt’s Method, Diazepam, Propranolal, ICH Guidelines

References