Asian Journal of Pharmaceutical Analysis

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New Simultaneous UV-Visible Spectrophotometric Methods for Estimation of Ofloxacin and Ketorolac Tromethamine in Ophthalmic Dosage Form


Affiliations
1 PES’s Modern College of Pharmacy (for Ladies), Borhadewadi, Dehu-Alandi Road, Moshi, Pune-412105, India
2 PES’s Modern College of Pharmacy (for Ladies), Borhadewadi, Dehu-Alandi Road, Moshi, Pune-412105
     

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New, simple, rapid and economic spectrophotometric methods have been developed for simultaneous estimation of Ofloxacin (OFLOX) and Ketorolac Tromethamine (KETO) in ophthalmic dosage form. For this, simultaneous Equation method (method A), Absorbance ratio method (Method B) and First order derivative spectroscopy (method C) were used. The method A involved measurement of absorbance at two wavelengths, 289 nm and 322 nm, max of OFLOX and KETO respectively. For method B, wavelengths 301 nm (isobestic point) and 289 nm (max of OFLOX) were used. In case of method C, wavelengths 262 nm (Zero crossing of OFLOX) and 274 nm (Zero crossing of KETO) were used for determination of KETO and OFLOX respectively. Beer's law obeyed in concentration range of 1.5-10.5 μg/ ml, and 2.5-17.5 μg/ ml for OFLOX and KETO respectively by all the methods. The proposed methods are recommended for routine analysis since they are rapid, simple, accurate and specific. Methods developed also showed no interference from Bezalkonium Chloride which is used as a preservative in the formulation. These methods were validated for linearity, precision and accuracy as per ICH guidelines.

Keywords

Ofloxacin, Ketorolac Tromethamine, Simultaneous, Spectrophotometry
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  • Budavari S, O’Neill MJ, Smith A, Heckelman PE, Kinneary JF. The Merck Index: An Encyclopedia of Chemicals, Drugs and Biologicals, 13th Edn., Merck and Co. Inc., Whitehouse Station, NJ, 2001, 6800.

  • Fabre D, Bressoll F, Kinowski JF , Bouvet O, Paganin F, Galtier M. A reproducible, simple and sensitive HPLC assay for determination of ofloxacin in plasma and lung tissue. Application in pharmacokinetic studies. Journal of Pharmaceutical Biomedical Analysis. 12(11); 1994: 1463-1469.

  • Du YX, Luo D, Wang QF. Determination of Ofloxacin in Human Serum by Reversed Phase HPLC. Journal of China Pharm Uni. 25; 1994: 32-35.

  • Basci NE, Hanioglu-kargi S, Soysal S, Bozkurt A, Kayaalp SO. Determination of Ofloxacin in Human Aqueous Humor by High Performance Liquid Chromatography with Fluorescence Detection. Journal of Pharmaceutical Biomedical Analysis. 15; 1997: 663-666.

  • Siva Kumar R, Kumar Nallasivan P, Saravanakumar S, Kandasamy CS, Venkarayanan R. Simultaneous RP-HPLC Estimation of Nitazoxanide and Ofloxacin in Tablet Dosage Forms. Asian Journal of Research in Chemistry. 2 (1); 2009: 43-45.

  • Shinde SR, Bhoir SI, Pawar NS, Yadav SB, Bhagwat AM. Simultaneous Estimation of Satranidazole and Ofloxacin in Tablet Dosage Form by High Performance Liquid Chromatography. E-Journal of Chemistry. 7(1); 2010:198-202.

  • Khandagle KS, Gandhi SV, Deshpande PB, Gaikwad NV. A Simple and Sensitive RPHPLC Method for Simultaneous Estimation of Cefixime and Ofloxacin in Combined Tablet Dosage Form. International Journal of Pharmacy and Pharmaceutical Sciences. 3(1); 2011;3 (1): 46-48.

  • Premanand DC, Senthilkumar KL, Senthilkumar V, Saravanakumar M, Thirumurthy R. A Validated RP-HPLC Method for Simultaneous Estimation of Nitazoxanide and Ofloxacin in Pharmaceutical Formulation. Der Chemica Sinica. 1 (2); 2010: 1-5.

  • Dhandapani B, Thirumoorthy V, Shaik HR, Rama kotaiah M, Anjaneyalu N. Method Development and Validation for the Simultaneous Estimation of Ofloxacin and Ornidazole in Tablet Dosage Form by RP-HPLC. International Journal of Pharmaceutical Sciences and Research. 1 (1); 2010: 78-83.

  • Singha R, Maithani1a M, Saraf K, Saraf S, Ram C. Simultaneous Estimation of Ciprofloxacin Hydrochloride, Ofloxacin, Tinidazole and Ornidazole by RP HPLC. European Journal of Analytical Chemistry. 4 (2); 2009: 161-167.

  • Dharuman J, Vasudevan M, Somasekaran KN, Dhandapania B, Ghodea PD, Thiagarajana M. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Ofloxacin and Tinidazole in Tablets. International Journal of Pharm Tech Research. 1 (2); 2009: 121-124.

  • Puranik M, Bhawsar DV, Rathi P, Yeole PG. Simultaneous determination of ofloxacin and ornidazole in solid dosage form by RP-HPLC and HPTLC techniques. Indian Journal of Pharmaceutical Sciences. 72 (4); 2010: 513-517.

  • Kraas E, Hirrle A. Determination of ofloxacin in biological fluids using HPLC with fluorimetric detection. Fresenius' Journal of Analytical Chemistry. 324 (3-4); 1986: 354.

  • Zhang XZ, Wen W, Jiang JY, Luo SD, Cai HS. First Order Derivative Spectrophotometry of Ofloxacin Gel. Chinese Journal of Pharmacy. 28; 1997: 314-315.

  • Liu JL. K-ratio and Differential Spectrophotometry of Ofloxacin and Metronidazole in Compound Ofloxacin Ear Drops. Chinese Journal of Pharmacy. 28; 1997: 514-515.

  • Jiang JY, Feng LZ, Li RL, Cai HS. Ultraviolet Spectrophotometry of Ofloxacin (OLX) Injection. Chinese Journal of Pharmacy. 27; 1996: 74-76.

  • Subrahmanyam CV, Reddy SE, Reddy MS. Analytical Method for Estimation of Ofloxacin in Urine. Indian Drugs. 33; 1996: 76-78.

  • N Murugesan, SC Mathur, Y Kumar, YK Rathore, PD Sethi. Spectrofluorometric Estimation of Ofloxacin in Tablet. Eastern Pharmacist. 35; 1992: 117-118.

  • Mathur SC, Kumar Y, Murugesan N, Rathore V, Sethi PD. Spectrophotometric Determination of Ofloxacin in Pharmaceutical Formulations. Indian Drugs. 29; 1992: 376-377.

  • Tuncel M, Atkosar Z. Determination of Ofloxacin in Tablet by Potentiometry and Conductometry. Pharmazie. 47;1992: 642- 643.

  • Prakash MS, Meena S. Fluorophotometric Determination of Ketororlac Tromethamine. Indian Drugs. 33; 1996: 149-151.

  • Irene T, Frances C, Martin K, Marian P, Anne W. HPLC Method for the Determination of Ketorolac in Human Plasma. Journal of Liquid Chromatography and Related Technologies. 19 (6); 1996: 957 – 967.

  • Chaudhary RS, Gangwal SS, Jindal KC, Khanna S. Reversedphase high-performance liquid chromatography of ketorolac and its application to bioequivalence studies in human serum. Journal of Chromatography B: Biomedical Sciences and Applications. 614 (1); 1993: 180-184.

  • Yang Guo-Ping, He Bin, Liu Shi-Kun. Determination of the content of ketorolac tromethamine in injection by HPLC. Chinese Journal of Hospital Pharmacy. 2003:12.

  • Reddy BP, Suryanarayana MV, Vemkatraman S, Krupadanam GL, Sastry CS Purity Evaluation of Ketorolac Tromethamine by HPLC. Indian Drugs. 30; 1993: 176-179.

  • Patri S, Patni AK, Iyer SS, Khuroo AH, Tausif M, Rana S. A Validated High-Performance Liquid Chromatography-Tandem Mass Spectrometric (LC-MS/MS) Method for Simultaneous Determination of R(+)-Ketorolac and S(-)-Ketorolac in Human Plasma and Its Application to a Bioequivalence Study. Chromatography Research International. 2011; 2011: 11.

  • Devarajan PV, Gore SP, Chavan SV. HPTLC Determination of Ketorolac Tromethamine. Journal of Pharmaceutical Biomedical Analysis. 22; 2000: 679-683.

  • Fegade JD, Mehta HP, Chaudhari RY, Patil VR. Simultaneous Spectrophotometric Estimation of Ofloxacin and Ketorolac Tromethamine in Ophthalmic Dosage Form. International Journal of Chem Tech Research. 1 (2); 2009:189-194.


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  • New Simultaneous UV-Visible Spectrophotometric Methods for Estimation of Ofloxacin and Ketorolac Tromethamine in Ophthalmic Dosage Form

Abstract Views: 913  |  PDF Views: 2

Authors

Vijaya Vichare
PES’s Modern College of Pharmacy (for Ladies), Borhadewadi, Dehu-Alandi Road, Moshi, Pune-412105, India
Vrushali Tambe
PES’s Modern College of Pharmacy (for Ladies), Borhadewadi, Dehu-Alandi Road, Moshi, Pune-412105
S. V. Joshi
PES’s Modern College of Pharmacy (for Ladies), Borhadewadi, Dehu-Alandi Road, Moshi, Pune-412105, India
S. N. Dhole
PES’s Modern College of Pharmacy (for Ladies), Borhadewadi, Dehu-Alandi Road, Moshi, Pune-412105, India

Abstract


New, simple, rapid and economic spectrophotometric methods have been developed for simultaneous estimation of Ofloxacin (OFLOX) and Ketorolac Tromethamine (KETO) in ophthalmic dosage form. For this, simultaneous Equation method (method A), Absorbance ratio method (Method B) and First order derivative spectroscopy (method C) were used. The method A involved measurement of absorbance at two wavelengths, 289 nm and 322 nm, max of OFLOX and KETO respectively. For method B, wavelengths 301 nm (isobestic point) and 289 nm (max of OFLOX) were used. In case of method C, wavelengths 262 nm (Zero crossing of OFLOX) and 274 nm (Zero crossing of KETO) were used for determination of KETO and OFLOX respectively. Beer's law obeyed in concentration range of 1.5-10.5 μg/ ml, and 2.5-17.5 μg/ ml for OFLOX and KETO respectively by all the methods. The proposed methods are recommended for routine analysis since they are rapid, simple, accurate and specific. Methods developed also showed no interference from Bezalkonium Chloride which is used as a preservative in the formulation. These methods were validated for linearity, precision and accuracy as per ICH guidelines.

Keywords


Ofloxacin, Ketorolac Tromethamine, Simultaneous, Spectrophotometry

References