Simultaneous Spectrophotometric Estimation of Naphazoline Nitrate and Hydrocortisone in Nasal Drops

Two new simple, sensitive, accurate, precise and economical spectrophotometric methods developed and validated for the simultaneous estimation of naphazoline nitrate and hydrocortisone in nasal drops. The first method is Q- absorbance ratio method. Q- Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Naphazoline nitrate and hydrocortisone show an isoabsorptive point at 264 nm in methanol. The second method is based on the derivative spectrophotometric method at zero crossing wavelengths. In this method the zero crossing point of naphazoline nitrate was selected at 277 nm and for hydrocortisone it was 290 nm. The linearity was obtained in the concentration range of 6.25-36.75 μg/ml for naphazoline nitrate and 5-30 μg/ml for hydrocortisone for both the above methods. These methods are simple, accurate and rapid, those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories. The suitability of these methods for the quantitative determination of the compounds was proved by validation.