Evaluation of In-vitro Anti-oxidant and Free Radical Scavenging Activities of Withania Somnifera and Aloe vera

In this study, In-vitro Antioxidant and free radical scavenging activity of Withania somnifera (WS) and Aloe vera (AV) extract were evaluated in series of in vitro assays. Anti-oxidants are vital substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress. In this present study we investigated anti-oxidant and free radical scavenging activity of Withania somnifera and Aloe vera by Hydroxyl radical scavenging activity, Hydrogen peroxide‐scavenging assay, Super oxide scavenging activity, Reducing power, Chelating capacity, Total antioxidant activity. The results showed that both the plant possesses excellent anti-oxidant and free radical scavenging activity. Screening of both the plant at different doses (100, 150 and 200 μg / ml) help to reveal the potential of individual plants. Withania somnifera shown better hydroxyl radical scavenging activity, reducing power and superoxide radical scavenging activity compare to Aloe vera. While Aloe vera possesses better chelating power then Withania somnifera. Both the plant possesses almost equivalent hydrogen peroxide scavenging activity while the total antioxidant capacity was found much better in Withania somnifera as compare to Aloe vera. The antioxidant activity of both the plant might be attributed to its polyphenolic content and other phytochemicals constituents. The findings of the present study suggest that all extracts could be a potential source of natural antioxidant that could have great importance as therapeutic agents.