Spectrophotometric Estimation of Cefepime and Captopril in Bulk and Dosage Forms using 4- Chloro- 7- Nitrobenzo-2-oxa-1,3-Diazole(NBD-Cl)

New simple, accurate and sensitive spectrophotometric method for determination of Cefepimeand Captopril in bulk and dosage forms.the developed method is based on the alkaline hydrolysis of the studied drugs and subsequent reactions of the resulting hydrolysates with NBD- Cl as a chromogenic reagent to form a yellow color measured at 401 nm. This mrthod could be used for their analysis in pure forms and in pharmaceutical formulations. Effect of reagent concentration, NaOH concentration, temperature, solvent, HClconcentraion, time of hydrolysis and time after addition of reagent on the absorption was studied. Beer's law was obeyed in the range of 8-120 μg/ml for cefepime and 2-24 μg/ml for Captopril. The proposed methods were applied for determination of Cefepimeand Captopril in pharmaceutical preparations and were validated when obtained results were compared with reference methods.