Spectrophotometric Fingerprinting Method for Unani formulation Hab-e-Azarakhi

Hab-e-Azarakhi is an important Unani formulation, is official in Unani Pharmacopoeia (formulary of Unani Medicine). The formulation is dispensed for the tone up the nervous system and liver. It is also useful for increase the appetite. Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Traditional medicines/herbal formulations. Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. The present study is an attempt to develop the fingerprint method for Hab-e-Azarakhi by spectrophotometric determination using Piperine as a standard, which is as an important content in formulation. The estimation was carried out with three laboratory batches and one marketed formulation by spectrophotometric approach at 342.6 nm. The UV spectrophotometric analysis, which is a simple, precise, and accurate method that can be considered as one of the quality control method for routine analysis.