In Vitro Anti-Oxidant Activity of the Various Extracts of Cassia auriculata L. Flower by UV Spectrophotometer

The present study was carried out in In-vitro antioxidant effects of the Petroleum Ether, Ethanol, and Methanolic flower extracts of Cassia auriculata L . The powder of flower of Cassia auriculata L was extracted sequentially with petroleum ether, ethanol and methanol using Soxhlet extractor and it was subjected to preliminary phytochemical studies for the identification of phytoconstituents by chemical tests. Antioxidant activity of the extract was evaluated by using Diphenyl picryl hydrazyl (DPPH) radical scavenging, and reducing power methods. The extracts of Cassia auriculata L had shown good DPPH (1, 1-diphenyl-2-picryl-hydrazyl) radical scavenging activity. BHA was used as standard antioxidant and positive control. The DPPH radical scavenging activity of the extracts was increased with the increasing concentration, the reducing power of extracts was carried out with ascorbic acid as a standard reducing agent. The Methanolic extract of Cassia auriculata L exhibited higher scavenging and reducing power than the other extracts. All the analysis was made with the use of UV Visible Spectrophotometer (Systronics 117, India). The observation suggest that the flower of Cassia auriculata L extracts has potent dose dependent anti oxidant activity, thus the Ethanobotanical claim of the plant being used in the cardio vascular disorder, muscle build up, nephro protection, anti inflammatory and as an aphrodisiac may be in part due to the antioxidant activity and also the presence of saponins and traces of Flavanoids ascertains that the Anti-oxidant activity.