Development of Novel HPTLC Method for Estimation of Qurcetine in Ocimum sanctum

HPTLC is a modern chromatographic technique in which the principle of TLC is sophisticated and automated by which the samples are accurately and precisely estimated which can be utilized for both qualitative and quantitative purpose.

Ocimum genus contains approximately 30 species of herbs and shrubs from the tropical and subtropical region of Asia, Africa and central and South America. However, the major place of diversity appears to be in Africa. The drug has numerous pharmacological activities like hypoglycemic, immunomodulatory, antistress, analgesic, antipyretic, antiinflammatory, antiulcerogenic, radio protective and antitumor. Major constituents of Ocimum sanctum includes eugenol, qurcetine etc. which are known to possess antifungal activity against candida albicans. The aim of the present study is to develop a novel High Performance liquid chromatographic (HPTLC) method for the quantitative estimation of flavonoid Qurcetine in hydroalcoholic extract of Ocimum sanctum.