Asian Journal of Pharmacy and Technology

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Nano Probes for mRNA Detection than using in-Situ Hybridization and Hence the Use of Nano Particles in Cancer Diagnosis and Therapy


Affiliations
1 Department of Biotechnology, Sri Venkateswara College of Engineering, Sriperumbudur, India
     

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In situ hybridization (ISH) is an efficient method for detecting the localization of mRNA. It has a lot of disadvantages that it doesn't provide information about the time dependent analysis also the information of post translational modification. They also suffer from serious disadvantage that they does not allow mRNA detection in living cells. Care should be taken in the extraction of the biomolecules from living cells that severe damage to the cells should be avoided.This review reveals the development of nano-structured oligonucleotide probes that has a predominant role in the detection of mRNA in the living cells. In particular, we describe methods that emphasize on nanotechnology in diagnosis and treatment for cancer.
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  • Nano Probes for mRNA Detection than using in-Situ Hybridization and Hence the Use of Nano Particles in Cancer Diagnosis and Therapy

Abstract Views: 191  |  PDF Views: 0

Authors

B. Mohammed Rizwan
Department of Biotechnology, Sri Venkateswara College of Engineering, Sriperumbudur, India
P. Sucharitha
Department of Biotechnology, Sri Venkateswara College of Engineering, Sriperumbudur, India
Jaibiba P.
Department of Biotechnology, Sri Venkateswara College of Engineering, Sriperumbudur, India

Abstract


In situ hybridization (ISH) is an efficient method for detecting the localization of mRNA. It has a lot of disadvantages that it doesn't provide information about the time dependent analysis also the information of post translational modification. They also suffer from serious disadvantage that they does not allow mRNA detection in living cells. Care should be taken in the extraction of the biomolecules from living cells that severe damage to the cells should be avoided.This review reveals the development of nano-structured oligonucleotide probes that has a predominant role in the detection of mRNA in the living cells. In particular, we describe methods that emphasize on nanotechnology in diagnosis and treatment for cancer.