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In-vitro Antioxidant activity of Marine Red Algae Gracilaria foliifera
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Seaweeds are potential as natural sources of antioxidants and have gained recent attention. The present study was made on antioxidant activity was performed by organic solvent extracts like methanol and aqueous of marine red algae Gracilaria foliifera (Forssk). In-vitro antioxidant analysis like DPPH, H2O2, OH, NO radical scavenging activity and total antioxidant activity also carried out by using standard procedures. In this study, radical scavenging activity was observed in different concentrations range from 100, 250, 500,750, 1000 μg/ml. The performances of scavenging activity of above extracts were comparing with standard ascorbic acid. An IC50 value of methanol and aqueous extract of DPPH radical were recorded at 679.27μg/ml and 801.12μg/ml, H2O2 radical were recorded at 790 μg/ml and 935.95μg/ml, hydroxyl radical at 687.92μg/ml and 159.56μg/ml respectively. Whereas nitric oxide the IC50 of methanol and aqueous were recorded at 812.75μg/ml and 1107.17μg/ml respectively. They were compared with IC50 value of standard ascorbic acid. In-vitro antioxidant results clearly indicated that the beneficial effect of G. foliifera exhibited significant value was compared with standard.
Keywords
Antioxidant, DPPH, Hydroxyl, Nitric Oxide, Hydrogen Peroxide.
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