Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Biofabrication of Silver Nanoparticles Using Cocculus hirsutus Leaf Extract and their Antimicrobial Efficacy


Affiliations
1 Department of Biotechnology, Vysya College, Salem- 636 103, Tamilnadu, India
2 Department of Biochemistry, Achariya Arts and Science College, Villianur, Pudhucherry-605 110, India
3 Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar- 608 002, Tamilnadu, India
     

   Subscribe/Renew Journal


In the recent decades, increased development of green synthesis of nanoparticles was inevitable because of its incredible applications in all fields of science. So we present a simple and eco-friendly biosynthesis of silver nanoparticles using Cocculus hirsutus leaf extract as reducing agent. The aqueous silver ions when exposed to leaf extract were reduced and resulted in silver nanoparticles whose average size was 139nm. The silver nanoparticles were characterized by UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-Ray Diffraction spectroscopy (XRD) and Scanning electron microscopy (SEM) techniques. Furthermore these biologically synthesized nanoparticles were found to be highly effective against human pathogens.

Keywords

Silver Nanoparticles, Cocculus hirsutus, Antimicrobial Activity
Subscription Login to verify subscription
User
Notifications
Font Size


  • Catauro M, Raucci MG, De Gaaetano FD, Marotta A. Antibacterial and bioactive silver-containing Na2O·CaO·2SiO2 glass prepared by sol-gel method. J Mater Sci- Mater Med. 2004; 15(7): 831-837.
  • Virender S, Yngard Ria A, Yekaterina L. Silver nanoparticles: Green synthesis and their antimicrobial activities. J Colloid Interface Sci. 2009; 145: 83-96.
  • Balantrapu K, Goia D. Silver nanoparticles for printable electronics and biological applications. J Mat Res. 2009; 24(9): 2828-2836.
  • Maria S, Barbara S, Jacek B. Electrochemical synthesis of silver nanoparticles. Electrochem Commun. 2006; 8(2): 227-230.
  • Begum NA, Mondal S, Basu S, Laskar RA, Mandal D. Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of black tea leaf extracts. Colloids Surf B. 2009; 71(1): 113-118.
  • Retchkiman-Schabes PS, Canizal G, Becerra-Herrera R, Zorrilla C, Liu HB, Ascencio JA. Biosynthesis and characterization of Ti/Ni bimetallic nanoparticles. Opt. Mater. 2006; 29: 95-9.
  • Gu H, Ho PL, Tong E,Wang L, Xu B. Presenting vancomycin on nanoparticles to enhance antimicrobial activities. Nano Lett. 2003; 3(9): 1261-3.
  • Ahmad Z, Pandey R, Sharma S, Khuller GK. Alginate nanoparticles as antituberculosis drug carriers: formulation development, pharmacokinetics and therapeutic potential. Ind J Chest Dis Allied Sci. 2005; 48: 171-6.
  • Gong P, Li H, He X, Wang K, Hu J, Tan W et al. Preparation and antibacterial activity of Fe3O4@Ag nanoparticles. Nanotechnology. 2007; 18: 604-11.
  • Karthick K, Kumaravel P, Hemalatha P, Thamaraiselvi L. Mechanistic aspects: Biosynthesis of silver nanoparticles from Proteus mirabilis and its antimicrobial study. Research Journal of Science and Technology. 2013, 5(2): 235-238.
  • Ravichandran Veerasamy, Tiah Zi Xin, Subashini Gunasagaran, Terence Foo Wei Xiang, Eddy Fang Chou Yang, Nelson Jeyakumar, Sokkalingam Arumugam Dhanaraj. Biosynthesis of silver nanoparticles using Mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society. 2011;15: 113-120.
  • Kasthuri J, Veerapandian S, Rajendiran N. Biological synthesis of silver and gold nanoparticles using apiin as reducing agent. Colloids Surf. B: Biointerf. 2009;68: 55-60.
  • Nestor ARV, Mendieta VS, Lopez MAC, Espinosa RMG, Lopez MAC, Alatorre JAA. Solvent less synthesis and optical properties of Au and Ag nanoparticles using Camiellia sinensis extract. Mater. Lett. 2008; 62, 3103-3105.
  • Gardea-Torresdey JL, Gomez E, Peralta-Videa JR, Parsons JG, Troiani H, Jose-Yacaman, M . Alfalfa sprouts: a natural source for the synthesis of silver nanoparticles. Langmuir. 2003;9: 1357- 1361.
  • Shankar SS, Ahmad A, Sastry M. Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol. Prog. 2003b;19: 1627-1631.
  • Li S, Qui L, Shen Y, Xie A, Yu X, Zhang L, Zhang Q. Green synthesis of silver nanoparticles using Capsicum annum L. extract. Green Chem. 2007;9: 852-858.
  • Kirtikar KR, Basu BD. Allahabad: Lalit Mohan Basu Publishers, 1987, p. 86.
  • Caius JF. The medicinal and poisonous plants of India. Jodhpur: Scientific Publishers, 1986: 166-171.
  • Usher G. A dictionary of plants used by man. 1st edition Delhi: CBS Publishers, 1984, p. 163.
  • El-Shabrawy AO, Schiff JR, Slatkin DJ, Dasgupta B, Ray AB, Tripathi VJ. Heterocycles 1984;22: 993.
  • Girach RD, Aminuddin, Siddiqui PA, Khan SA. Int J Pharmacog.1994;32: 274.
  • Khandelwal N, Singh A, Jain D, Upadhyay MK and Verma HN. Green synthesis of silver nanoparticles using Argimone mexicana leaf extract and evaluation of their antiomicrobial activities. J. Nanomater. Biostrct. 2010; 5: 483-489.
  • Elumalai EK, Prasad TNVK, Hemachandran J, Viviyan therasa S, Thirumalai T, David E. J. Phar. Sci. Res. 2010;2: 549.
  • Farooqui MDA, Chauhan PS, Krishna moorthy P, Shaik J. Dig. J. Nano. Bio. 2010;5: 43.
  • Sathyavathi R, Krishna MB, Rao SV, Saritha R and Rao DN. Biosynthesis of Silver Nanoparticles Using Coriandrum Sativum leaf Extract and their application in Nonlinear Optics. Adv. Sci. Lett. 2010; 3:1-6.
  • Sondi I, Salopek Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram negative bacteria. J. Colloids Interface Sci. 2004; 2275: 177-182.
  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramfrez JT, Yacaman, MJ. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16: 2346-2353.

Abstract Views: 976

PDF Views: 0




  • Biofabrication of Silver Nanoparticles Using Cocculus hirsutus Leaf Extract and their Antimicrobial Efficacy

Abstract Views: 976  |  PDF Views: 0

Authors

C. Thiruppathi
Department of Biotechnology, Vysya College, Salem- 636 103, Tamilnadu, India
P. Kumaravel
Department of Biotechnology, Vysya College, Salem- 636 103, Tamilnadu, India
R. Duraisamy
Department of Biotechnology, Vysya College, Salem- 636 103, Tamilnadu, India
A. K. Prabhakaran
Department of Biotechnology, Vysya College, Salem- 636 103, Tamilnadu, India
T. Jeyanthi
Department of Biochemistry, Achariya Arts and Science College, Villianur, Pudhucherry-605 110, India
R. Sivaperumal
Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar- 608 002, Tamilnadu, India
P. A. Karthick
Department of Biotechnology, Vysya College, Salem- 636 103, Tamilnadu, India

Abstract


In the recent decades, increased development of green synthesis of nanoparticles was inevitable because of its incredible applications in all fields of science. So we present a simple and eco-friendly biosynthesis of silver nanoparticles using Cocculus hirsutus leaf extract as reducing agent. The aqueous silver ions when exposed to leaf extract were reduced and resulted in silver nanoparticles whose average size was 139nm. The silver nanoparticles were characterized by UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-Ray Diffraction spectroscopy (XRD) and Scanning electron microscopy (SEM) techniques. Furthermore these biologically synthesized nanoparticles were found to be highly effective against human pathogens.

Keywords


Silver Nanoparticles, Cocculus hirsutus, Antimicrobial Activity

References