Simultaneous Spectrophotometric Estimation of Glimepiride and Pioglitazone HCl in Combined Dosage Form Using Absorbance Correction Method

A simple, sensitive, rapid, accurate and precise UV-spectrophotometric (absorbance correction) method for simultaneous estimation of glimepiride and pioglitazone HCl in pharmaceutical dosage form was developed and validated for accuracy, precision, ruggedness, linearity and range. The wavelengths selected for estimation of drugs were 270.0 nm for pioglitazone HCl and 230.0 nm for glimepiride. Linearity for detector response for glimepiride and pioglitazone HCl were in the range of 2-50 μg/ml and 10-170 μg/ml respectively. The method gives results of high accuracy and high recovery of 100.34 ± 0.25 and 99.54 ± 0.47 for glimepiride and pioglitazone HCl respectively. % R.S.D. values for the marketed formulation analysis were found to be less than 2 which indicated good precision and reproducibility of the method. Commercial capsule formulation was successfully analyzed using the developed method.