Method Development and Validation of Brimonidine Tartrate by High Performance Liquid Chromatography

A rapid, simple and accurate RP-HPLC method was developed and validated for the determination of brimonidine tartrate. The analysis was performed by using Kromasil C-18 column in Gradient mode. Methanol was used as an organic phase while aqueous phase comprising of potassium dihydrogen phosphate buffer (10 mM) by adjusting to pH 3.0. The flow rate and detection wavelength were 1ml/min and 246 nm, respectively. The retention time for brimonidine tartrate was found to be 10.51 min. Developed method was validated with respect to the parameters such as linearity, precission, accuracy, system suitability, limit of detection, limit of quantitation and robustness.