Simultaneous Estimation of Paracetamol, Caffeine, Pseudoephedrine Hydrochloride, Dextromethorphan Hydrobromide and Loratadine in Tablet Dosage form by RP-HPLC

A simple, selective, rapid, precise and economical RP HPLC method has been developed for the determination of Paracetamol, Caffeine, Pseudoephedrine Hydrochloride, Dextromethorphan Hydrobromide and Loratadine in tablet formulation. The analysis was resolved by using a mobile phase (Sodium heptane sulphonate buffer solution and Acetonitrile in the ratio of 95:5) at a flow rate of 1ml/min on an gradient consisting of Shimadzu LC 2010 HPLC system on variable wavelength UV detector using, Inertsil ODS 3V (4.6 mm x 5 cm, 3 μm) column at a wavelength of 205 nm. The retention time were found to be Paracetamol (3.5min), Caffeine (5.5min), Pseudoephedrine HCl (8min), Dextromethorphan HBr (14min), Loratadine (15min). The percent recovery of Paracetamol, Caffeine, Pseudoephedrine Hydrochloride, Dextromethorphan Hydrobromide and Loratadine were found to be in between 98% to 102%. The developed method was simple, precise, accurate and reproducible and therefore suitable for routine analysis of drugs in tablet dosage form.