Development and Validation of Phyllanthin by HPTLC in Hepatoprotective Polyherbal Tablet Dosage Form

A simple, precise, accurate, rapid and reproductive HPTLC procedure was developed for development and validation of phyllanthin in tablet dosage form at a single wavelength. The mobile phase used was a combination of Toluene-Ethyl acetate [9:1 V/V]. The detection of the combined dosage form was carried out at 200nm. The Rf value of standard phyllanthin was 0.83 ± 0.04 and matches to the phyllanthin present in polyherbal tablet. The phyllanthin content of tablet was determined and it was found to be 0.178 % w/w ±0.0016.The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.