Electrochemical Analysis of Fatty Acids Obtained from the Natural Resource Seed of Perilla frutescens

Perilla frutescens is grown as an oil seed crop in Sikkim. The saponifiable portion of fixed oil from the seeds of the tree has been analyzed using polarographic technique, which has revealed the presence of saturated fatty acids with E_1/2/E_p values equal to 1: -0.56/-0.57 V, and 2: -0.62/-0.64 V vs S.C.E., corresponding to Palmitic acid (8.2%) and Stearic acid (5.73%) respectively. Whereas the unsaturated fatty acid mixture produced three waves Fig. III/IVB/IVB1 with E_1/2/E_p values equal to 1: -0.08/-0.08 V, 2: -0.30/-0.32 V and 3: -0.48/-0.48 V Vs S.C.E., corresponding to Linolenic acid (64.4%), Linoleic acid (13.5%) and Oleic acid (8.06%) respectively. The method has been successfully applied for the qualitative as well as quantitative analysis of fatty acids. Each of fatty acid present in the oil produced is well defined polarographic DCP and DPP Signals in 0.1M TMAB and 0.1M Cd²⁺ used as a supporting electrolyte as pH 4.0 ± 0.2.