Quantification of Quercetin and Rutin in Arjunarishta Prepared by Traditional and Modern Methods by Validated HPTLC Densitometry

Arjunarishta, also known as Parthadhyarishta, is a polyherbal hydro alcoholic formulation and is advised as a choice of remedy in cardiovascular disorders. A simple, precise and accurate HPTLC method has been established for the determination of quercetin and rutin in Arjunarishta-T and Arjunarishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ, specificity, robustness and ruggedness. The amount of quercetin in Arjunarishta-T, M and its marketed formulation was found to be 0.00260, 0.00234 and 0.00252% w/w respectively while rutin was found to be 0.00585, 0.00553 and 0.00568% w/w respectively. This is the first report for the quantification of quercetin and rutin in Arjunarishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of quercetin and rutin from Arjunarishta.