Biological Cytotoxicity Evaluation of Sulfonamide Derivatives as Anti-Lung and Anti-Breast Cancer Activity

The purpose of this research work was to evaluate the cytotoxic activity of Sulfonamide derivatives against the panel of lung cancer and breast cancer cell lines, which were MDAMB-453, MCF-7, MDAMB-468, NCI-H23, and NCIH522 with one normal cell lines, which was HEK-293T (Human embryonic kidney cell line); by comparing the results with the standard drug Doxorubicin. For primary screening, sulfonamide derivatives were screened at 100 μM concentration against all cell lines with the use of XTT based cytotoxicity assay and the compounds which were found to be active as a anti-cancer and those with lower IC50 value below 60 M were again subjected to MTT assay for confirmation. From the results, Compound No. 1 having substitution with chlorine group showed cytotoxicity against panel of breast cancer cell lines (IC50 = 48.39 μM in MCF-7, IC50 = 36.86 μM in MDA-MB453 and IC50 = 38.52 μM in MDA-MB468) but it is found to be inactive against lung cancer cell lines with no activity in normal cell line.