Development and Validation of High Performance Liquid Chromatographic Method for Estimation of Deflazacort in Pharmaceutical Formulation

A simple, selective, rapid and precise reverse phase HPLC method has been developed for the estimation of Deflazacort in pharmaceutical dosage form. A Hypersil BDS C₁₈ (250 mm X 4.6mm) column was used for separation. The mobile phase was ammonium acetate buffer solution: acetonitrile (50:50 v/v). Flow rate 1.0ml/min with detection at 254 nm. The retention time of Deflazacort was 6.03min. Linearity obtained in the concentration range of 20-100μg/ml with correlation coefficient of 0.998.The result of the analysis were validated statistically and recovery studies confirmed by the accuracy of the proposed method. The proposed RP-HPLC method for the estimation of Deflazacort in dosage form is accurate, precise, linear, robust, simple, rapid and selective.