Phytochemical Screening and In-vitro Antioxidant Activity of Cissus quadrangualris

Cissus quadrangualris is a medicinal herb used in traditional medicine of India as fracture healing process, asthma, cardiac problems, tumor and skin diseases. Cissus quadrangualris stem extracted with five solvents (Petroleum ether, chloroform, ethylacteate, methanol and water). Antioxidant activity of methanol extract of Cissus quadrangualris stem was studied for its free radical scavenging property on in-vitro models: ABTS^.+ and Superoxide anion radical scavenging activity comparable with that of reference antioxidant butylated hydroxytoluene (BHT).The extract showed good dose dependent free radical scavenging property in ABTS^.+ and Superoxide anion radical scavenging assay. In general, the methanol extract exhibited good antioxidant activity in ABTS^.+ and Superoxide anion(81.49±0.03 μg/ml) and (71.19±0.03 μg/ml) but were significantly lower than the synthetic antioxidant (84.23±0.04 μg/ml) and (79.23±0.01 μg/ml). The IC₅₀ values of ABTS^.+ and Superoxide anion radical scavenging activity were found to be (179.6±0.03 μg/ml), (190±0.04 μg/ml) and that of the standard BHT was (150.0±0.02 μg/ml) and (173.3±0.06 μg/ml). Phytochemical Screening disclosed the presence of alkaloids, flavonoids, saponins, terpenoids, steroids, tannins, cardioglycosides, amino acids and proteins. The result suggests that the methanol extracts of Cissus quadrangualris stem have potent antioxidant activity against free radicals.