Determination of Metoprolol Succinate and Atorvastatin Calcium in Capsules Using RP-HPLC

A rapid sensitive and specific RP-HPLC method involving UV detection was developed and validated for the simultaneous determination and quantification of metoprolol and atorvastatin. Chromatography was carried out on a Zorbax X - C18 (150 X 4.6mm, 5 μm) column using buffer and acetonitrile (50: 50) as mobile phase at a flow rate of 1 ml/min and effluent was monitored at 280 nm. The assay was linear over 25 - 150 μg/ml for metoprolol and atorvastatin. The retention times of metoprolol and atorvastatin were found to be 5.265 and 6.856 min, respectively. The percentage recovery obtained was 100.8 and 100.61 % respectively.